摘要
以红米为原料,采用8种大孔树脂对红米色素进行静态吸附和解吸试验,筛选出合适的树脂型号,确定用AB-8对红米色素进行纯化,得到AB-8吸附红米色素平衡时间为4h,解吸终点时间为120min。通过单因素和正交试验得到了最佳动态吸附工艺参数,即上柱液流速1.0ml/min、上柱液温度20℃、上柱液pH3.0、上柱液质量浓度1.5mg/ml;最佳动态解吸条件为:乙醇体积分数75%,pH1.5,洗脱流速为1.0ml/min,解吸率最高。在此条件下,纯化的色素呈暗红色,经过纯化后色价为9.79,比未纯化红米色素提高了30%。
With red rice as raw material,using 8kinds of macroporous resin for red rice pigment static adsorption and desorption test to select the suitable resin,AB-8resin was determined for purification of red rice pigment.The equilibrium time of AB-8adsorption red rice pigment was 4h,the desorption end time was 120 min.Through single factor and orthogonal experiment,the optimal dynamic adsorption process parameters were as：the upper column liquid flow rate of 1.0ml/min,the column temperature of 20℃,pH3.0and the column concentration of 1.5mg/ml;the optimal dynamic desorption conditions were ethanol concentration 75%,ethanol pH 1.5,elution flow rate of 1.0ml/min,desorption rate was the highest.Under the selected condition,the pigment was dark red,and the color value was 9.79 after purification,increased by 30%.
出处
《粮食与饲料工业》
CAS
2016年第4期40-43,47,共5页
Cereal & Feed Industry
基金
辽宁省普通高等教育本科教学改革研究项目(UPRP20140116)
辽宁省教育科学规划课题(JG14DB310)
关键词
大孔树脂
红米色素
吸附
解吸
纯化
macroporous resin
red rice pigment
adsorption
desorption
purification
