摘要
目的改进GB/T5009.149-2003《食品中栀子黄的测定第一法高效液相色谱法》的方法。方法样品经甲醇:甲酸(6:4,V:V)洗脱,采用Angilent Extend C_(18)反相色谱柱(4.6 mm×250 mm,5μm),以甲醇:0.2%氨水(50:50,V:V)为流动相(等度洗脱),流速为1.0 mL/min,进样量20μL,经配有二极管阵列检测器(DAD)的高效液相色谱仪(high performance liquid chromatograph,HPLC)进行测定。结果本方法检出限为3.2 mg/kg,栀子黄中藏花酸和藏花素峰面积的相对标准偏差分别为1.6%、2.5%,在5~50μg/mL范围内有较好的线性,Y=1.34X-0.54,r^2=0.9998,不同浓度的回收率在91.2%~98.4%之间。结论本方法简单、高效、准确度高,回收率和重现性良好,适用检测的食品类别范围较宽,具有推广应用价值。
Objective To improve the method of GB/T 5009.149-2003 Determination ofcrocin in food by high performance liquid chromatography.Methods After eluted with methanol：formic acid（6：4,V：V）,samples were determined by high performance liquid chromatography（HPLC） equipped with diode array detection（DAD）,using Agilent extend C_（18） reversed-phase chromatographic column（4.6 mm×250 mm,5 μm）,with methanol：0.2%ammonia water（50：50,V：V） as mobile phase（isocratic elution） at a flow rate of 1.0mL/min,and injection volume of 20 μg.Results The detection limit of the method was 3.2 mg/kg.The RSDs of the peak area of crocetin and crocin in gardenia yellow were 1.6%and 2.5%,respectively.And they had a good linearity in the range of 5~50 μg/mL,Y=1.34X-0.54,r^2=0.9998.The recoveries were between91.2%~98.4%.Conclusion This method is simple,efficient,accurate,and has good recovery rate and reproducibility,which is suitable for the detection of a wide range of food categories,and has the value of popularization and application.
出处
《食品安全质量检测学报》
CAS
2016年第3期917-920,共4页
Journal of Food Safety and Quality
关键词
栀子黄
高效液相色谱法
栀子苷
藏花酸
藏花素
gardenia yellow
high performance liquid chromatography
geniposide
crocetin
crocin
