摘要
目的分析云浮市麻疹实验室检测情况,探讨麻疹发病和流行特点,提高实验室检测水平,指导麻疹防控。方法采集麻疹疑似病例血清,开展麻疹Ig M抗体检测,同时采集病原学标本检测病毒核酸,并对麻疹病毒核酸阳性标本进行病毒分离。结果全年共检测血清标本123份,麻疹Ig M阳性85份,阳性率为69.1%;麻疹PCR阳性80份。共分离到21株麻疹病毒,均为H1a基因亚型。麻疹检测阳性病例主要分布在<5岁年龄段人群,发病时间主要在6月-8月。出疹后3 d内采集的标本病毒分离率高于3 d后采集的标本,差异有统计学意义(χ~2=4.62,P<0.05),于采集当天送检的标本病毒分离率高于采样后1 d^3 d和>3 d送检的标本,差异有统计学意义(χ~2=8.35,P<0.05)。结论云浮市目前流行的麻疹野病毒为H1a基因亚型,PCR检测方法是传统血清学检测方法的补充,及早采样和送样是提高病毒分离率的关键。
Objective To analyze laboratory test data of measles in Yunfu. To investigate the incidence and epidemic characteristics of measles,improve the level of laboratory testing,so as to provide guidance for the prevention and control of measles.Methods Serum specimens from suspected measles cases were collected and measles Ig M antibodies were tested. At the same time,the samples were collected to detect viral nucleic acids,and the nucleic acid positive samples of measles virus were isolated from the virus. Results A total of 123 specimens were collected. 85 were detected as measles with a positive rate of69. 1%. 80 specimens were detected as positive. 21 strains of measles virus were isolated,which were identified as H1 a genotype. The age of measles cases were mainly distributed under 5 years old. The epidemic peak was from June to August. Virus isolation rate in specimens collected within 3 days after the rash was higher than that after 3 days,with the difference statistically significant（ χ-2= 4. 62,P〈0. 05）. The virus isolation rate of specimens sent for test on the same day was higher than that in1 d- 3 d or after 3 days,with the difference statistically significant（ χ-2= 8. 35,P〈0. 05）. Conclusion The wild measles virus in Yunfu was H1 a genotype. PCR detection method is a complement to the traditional methods of serological testing. Sampling and submission timely is the key to improve the rate of virus isolation.
出处
《中国卫生检验杂志》
CAS
2016年第7期959-961,共3页
Chinese Journal of Health Laboratory Technology
关键词
麻疹
麻疹IGM抗体
核酸检测
病毒分离
Measles
Measles IgM antibody
Nucleic acid detection
Virus isolation
