摘要
目的探讨白藜芦醇(RES)对佐剂性关节炎(AA)大鼠关节炎症及肾脏损伤的治疗效果及其分子机制。方法将56只SD大鼠随机分为空白组(n=8),溶剂组(n=8),AA模型组(n=10),AA+RES低剂量组(n=10,5 mg/kg/d),AA+RES中剂量组(n=10,5 mg/kg/d),AA+RES高剂量组(n=10,5 mg/kg/d)。采用弗氏完全佐剂建立AA大鼠模型,并通过测量足爪肿胀、全身炎症反应及关节炎症评分的方法进行半定量评价。生化法检测各组大鼠肾脏生化功能改变,检测各组大鼠血清肌酐(Cr)、尿素氮(BUN)及肾脏病理学改变,检测血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)、一氧化氮(NO)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)及过氧化氢酶(CAT)含量的改变。结果 RES治疗组的关节病理学评分明显低于模型组和溶剂组,生化检测发现Res能改善肾脏BUN、Cr的表达水平,RES能够明显改善AA大鼠体内脂质过氧化水平。结论 RES能够明显改善AA大鼠的关节炎症和肾脏损伤程度,其机制可能为RES降低脂质过氧化水平。
Objective To investigate the treatment effect of resveratrol and its molecular mechanism in adjuvant arthritis rats. Methods 56 SD rats were randomly divided into control group（ n = 8）,solvent group（ n = 8）,AA model group（ n = 10）,AA ＋ RES low dose group（ n = 10,5mg/kg/d）,AA ＋ RES mid dose group（ n = 10,5mg/kg/d）,AA ＋ RES high dose group（ n = 10,5mg/kg/d）. Using Freund＇s complete to established AA rat model,and by measuring paw swelling,systemic inflammation and arthritis scoring method for semiquantitative evaluation. D. We detected rat renal function changes by biochemical methods and the change of superoxide dismutase（ SOD）,malondialdehyde（ MDA）,nitric oxide（ NO）,glutathione（ GSH）,glutathione peroxidase（ GSH-PX） and catalase（ CAT） content in the serum. Results The joint pathology scores in RES treatment groups were significant lower than the model group and solvent group,meanwhile,RES improved the BUN and Cr expression by biochemical detection. RES significantly improved lipid peroxidation in vivo in rats AA. Conclusions RES could alleviated arthritis and kidney damage in AA rats which may have relationship with reduction of lipid peroxidation.
出处
《齐齐哈尔医学院学报》
2016年第8期981-984,共4页
Journal of Qiqihar Medical University
基金
安徽高校自然科学研究重点项目(KJ2015A350)
