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不同好氧颗粒污泥中微生物群落结构特点 被引量:28

Characteristics of microbial community structure in different aerobic granular sludge
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摘要 为了探讨活性污泥好氧颗粒化过程对微生物种群的影响、不同底物及不同颗粒化方法培养的好氧颗粒污泥中微生物群落结构的差异,以接种污泥、模拟废水好氧颗粒污泥和分别投加粉末活性炭和硅藻土的实际生活污水好氧颗粒污泥为研究对象,利用PCR-DGGE对比分析了接种污泥和好氧颗粒污泥中的微生物群落结构.结果表明:活性污泥好氧颗粒化过程会减少微生物种群多样性,影响颗粒污泥稳定性的细菌被淘汰,而聚磷菌、反硝化菌、难降解有机物降解菌等污水处理功能微生物都在颗粒化过程中得到保留.活性污泥好氧颗粒化过程中能够实现亚硝化细菌(AOB)一定程度的富集.与接种活性污泥相比,好氧颗粒污泥中AOB的多样性指数与均匀性指数均有提高.好氧颗粒污泥中的优势菌群主要分布于变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和未培养菌(uncultured bacterium).其中AOB均属于β-Proteobacteria的亚硝化单胞菌属(Nitrosomonas). In order to investigate the influences of activated sludge aerobic granulation process on microbial diversity and the differences of microbial community structure in aerobic granular sludge(AGS) which was cultivated by different carbon sources or different granulation methods, the microbial community structure of seed sludge and AGS(AGS with artificial wastewater, AGS with real domestic wastewater by adding diatomite and powered activated carbon respectively) were analyzed by PCR-DGGE. The activated sludge aerobic granulation process led to a low microbial diversity. The bacteria which might impact the stability of granular sludge were eliminated. However, the bacteria which were capable of phosphorus accumulation, denitrification or bio-degradation of refractory organic substances were retained. In the activated sludge aerobic granulation process the ammonia oxidation bacteria(AOB) were enriched to a certain extent. Compared with the seed activated sludge, the diversity index and evenness index of AOB were increased. The dominant bacterial communities in AGS were Proteobacteria, Bacteroidetes and uncultured bacterium. The dominant AOB in AGS were all belonged to Nitrosomonas.
出处 《中国环境科学》 EI CAS CSCD 北大核心 2016年第4期1136-1144,共9页 China Environmental Science
基金 国家水体污染控制与治理科技重大专项资助项目(2014ZX07201-011)
关键词 好氧颗粒污泥 微生物群落结构 PCR-DGGE aerobic granular sludge microbial community structure PCR-DGGE
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参考文献16

  • 1Moy B Y P, Tay J H, Toh S K, et al. High organic loading influences the physical characteristics of aerobic sludge granules[J]. Letters Applied Microbiology, 2002,34(6):407-412.
  • 2王杰,彭永臻,杨雄,王淑莹.不同碳源种类对好氧颗粒污泥合成PHA的影响[J].中国环境科学,2015,35(8):2360-2366. 被引量:22
  • 3Yang S F, Tay J H, Liu Y. A novel granular sludge sequencing batch reactor for removal of organic and nitrogen from wastewater[J]. Journal of Biotechnology, 2004,106(1):77-86.
  • 4Ni B J, Xie W M, Liu S G, et al. Granulation of activated sludge in a pilot-scale sequencing batch reactor for the treatment of low-strength municipal wastewater[J]. Water Research, 2008, 43(3):751-761.
  • 5Heijnen J J, Loosdrecht M C M V, Mulder R, et al. Development and scale-up of an aerobic biofilm air-lift suspension reactor[J]. Water Science and Technology, 1993,27(5/6):253-261.
  • 6高阳,徐晓晨,徐光景,杨凤林,靳文尧.活性炭为载体的好氧颗粒污泥培养及性能研究[J].水资源与水工程学报,2012,23(5):25-28. 被引量:5
  • 7Wagner M, Loy A. Bacterial community composition and function in sewage treatment systems[J]. Current Opinion in Biotechnology, 2002,13(3):218-227.
  • 8Nguyen H T, Nielsen J L, Nielsen P H.‘Candidatus Halomonas phosphatis’, a novel polyphosphate-accumulating organism in full-scale enhanced biological phosphorus removal plants[J]. Environmental Microbiology, 2012,14(10):2826-2837.
  • 9Ebm D, Vybiral D, Koblízek M P, et al. Erythrobacter citreus sp nov., a yellow-pigmented bacterium that lacks bacteriochlorophyll a, isolated from the western Mediterranean Sea[J]. International Journal of Systematic and Evolutionary Microbiology, 2002,52(5):1655-1661.
  • 10毛跃建.废水处理系统中重要功能类群Thauera属种群结构与功能的研究[D].上海:上海交通大学,2009.

二级参考文献49

  • 1王芳,于汉英,张兴文,张捍民,刘毅慧,杨凤林.进水碳源对好氧颗粒污泥特性的影响[J].环境科学研究,2005,18(2):84-88. 被引量:22
  • 2王迪,杨凤林,周军,安鹏.碳源对好氧颗粒污泥物理性状及除磷性能的影响[J].中国给水排水,2007,23(5):85-89. 被引量:11
  • 3张丽丽,陈效,陈建孟,蔡伟民.胞外多聚物在好氧颗粒污泥形成中的作用机制[J].环境科学,2007,28(4):795-799. 被引量:54
  • 4Siripong S,Rittmann B E.Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants[J].Water Research,2007,41(5):1110-1120.
  • 5Qin Y Y,Zhang X W,Ren H Q,et al.Population dynamics of ammonia-oxidizing bacteria in an aerated submerged biofilm reactor for micropolluted raw water pretreatment[J].Applied Microbiology and Biotechnology,2008,79(1):135-145.
  • 6Hallin S,Lydmark P,Kokalj S,et al.Community survey of ammonia-oxidizing bacteria in full-scale activated sludge processes with different solids retention time[J].Journal of Applied Microbiology,2005,99(3):629-640.
  • 7Limpiyakorn T,Shinohara Y,Kurisu F,et al.Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo[J].Ferns Microbiology Ecology,2005,54(2):205-217.
  • 8Lim J,Do H,Shin S G,et al.Primer and probe sets for group-specific quantification of the genera Nitrosomonas and Nitrosospira using real-time PCR[J].Biotechnology and Bioengineering,2008,99(6):1374-1383.
  • 9Eschenhagen M,Schuppler M,Roske I.Molecular characterization of the microbial community structure in two activated sludge systems for the advanced treatment of domestic effluents[J].Water Research,2003,37(13):3224-3232.
  • 10Osbom A M,Moore E R B,Timmis K N.An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics[J].Environmental Microbiology,2000,2(1):39-50.

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