摘要
目的:观察中药复方安肠愈疡汤对溃疡性结肠炎(Ulcerative Colitis,UC)大鼠结肠组织中IL-6、IL-13含量及NF-κB表达的影响,以探讨其作用机制。方法:将90只Wistar大鼠随机分为6组(空白对照组,模型对照组,安肠愈疡汤低、中、高剂量组和美沙拉嗪组)。除空白组外,其他各组均应用三硝基苯磺酸(TNBS)制备UC大鼠模型,造模成功后,按照组别分别给予不同剂量的实验药物,连续给药3周后,进行结肠组织肉眼形态评分,同时采用ELISA法检测结肠组织中IL-6、IL-13含量,免疫组化法检测NF-κB蛋白表达。结果:安肠愈疡汤中、高剂量组及美沙拉嗪组大鼠结肠黏膜均有不同程度的炎症修复、溃疡愈合(P<0.01或P<0.05),其中高剂量组及美沙拉嗪组明显优于中、低剂量组,但2组比较无统计学意义(P>0.05);在IL-6方面,安肠愈疡汤低、中、高剂量组及美沙拉嗪组的含量均不同程度降低(P<0.05或P<0.01),其中,高剂量组及美沙拉嗪组明显优于中、低剂量组(P<0.01),但2组之间无统计学意义(P>0.05);在IL-13方面,安肠愈疡汤中、高剂量组及美沙拉嗪组的含量均不同程度升高(P<0.01或P<0.05),尤以高剂量组效果最好,与美沙拉嗪组比较有差异有统计学意义(P<0.05);在NF-κB表达方面,安肠愈疡汤中、高剂量组及美沙拉嗪组均有不同程度降低(P<0.05或P<0.01),其中高剂量组效果最好,但与美沙拉嗪组相比无统计学意义(P>0.05)。结论:安肠愈疡汤具有较好的抗炎和促进溃疡修复的作用,可能是通过下调致炎因子IL-6,上调抗炎因子IL-13,同时降低NF-κB表达,从而起到治疗UC的作用。
Objective： To explore the mechanism of Anchang Jieyu decoctiona by detecting its effect on the expression of IL-6,IL-13 and NF- k B in rats with ulcerative colitis（ UC）. Methods： Ninety Wistar rats were randomly divided into six groups（ blank control group,model control group,Anchang Jieyu decoctiona low,medium,high dose group and Solfasalazine（ 5-ASA） group）.Except for the blank group,the other groups were given trinitrobenzene sulfonic acid（ TNBS） to produce rat model of UC. Different groups were given different dose of drugs respectively. After 3 weeks＇ administration,colon tissue of the eye morphology scores,IL-6,IL-13 content,and NF-κB protein expression were detected. Results： Rats in Anchang Jieyu decoctiona low,medium,high dose group and 5-ASA group showed varying degrees of improvement and ulcer healing（ P〈0. 01 or P〈0. 05）,of which the high dose group and the 5-ASA were superior to others,but comparison between the two groups showed no statistical significance（ P〉0. 05）; In level of IL-6,TCM low,medium and high dose group and the 5-ASA group were differently lower（ P〈0. 05 or P〈0. 01）. Among them,the high dose group and the 5-ASA were superior to middle and low dose group（ P〈0. 01）,but no statistical significance had showed between the two groups（ P〉0. 05）; In level of IL-13,TCM high dose group and 5-ASA group were differently increased（ P〈0. 01 or P〈0. 05）,especially in high dose group. Besides,compared with 5-ASA group,there was significant differences（ P〈0. 05）; In level of NF- k B,TCM medium,high dose group and 5-ASA group have declined（ P〈0. 05 or P〈0. 01）,in which the effect of high dose group performed the best. While compared with 5-ASA group,there were no statistical significance（ P〉0. 05）. Conclusion： Anchang Jieyu decoction performs better in anti-inflammation and ulcer repair promotion. This may through down regulating pro-inflammatory cytokines IL-6,up regulating anti-inflammatory cytokines IL-13,and inhibiting the expression of NF-κB,which contribute a lot to the treatment of UC.
出处
《世界中医药》
CAS
2016年第1期113-116,共4页
World Chinese Medicine
基金
山东省自然科学基金(编号:ZR2012HL11)--"溃疡性结肠炎大鼠结肠组织NF-κB
TLR-4表达与其损伤关系及中药复方干预的研究"
