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不同浓度玻璃化冷冻保护液及冷冻载体对驴卵母细胞成熟率的影响 被引量:1

Effect of Different Vitrificated Cryopreservation Solutions and Loading Devices on in vitro Maturation Rate of Donkey Oocytes
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摘要 为了比较不同冷冻液及冷冻载体对驴卵母细胞成熟率的影响,试验设计4个浓度梯度(Ⅰ、Ⅱ、Ⅲ、Ⅳ)冷冻液和3种冷冻载体分别对生发泡(germinal vesicle,GV)期驴的卵母细胞进行玻璃化冷冻,对解冻后的卵母细胞进行体外成熟,通过比较卵母细胞的成熟率反映其冷冻效果。结果表明,冷冻液Ⅱ的卵母细胞成熟率(28.13%)显著(P<0.05)高于其他3组,但显著(P<0.05)低于正常组(51.77%),在不同冷冻载体对驴卵母细胞成熟率的影响试验中,使用开放式拉长塑料细管(OPS)的卵母细胞成熟率(28.13%)显著(P<0.05)高于普通玻璃细管组(11.67%),但与GMP管组(23.53%)差异不显著(P>0.05)。 The aim of the present study was to evaluate the effect of different vitrificated cryopreservation solutions and loading devices on in vitro maturation rate of donkey oocytes. Four concentration gradient of solutions( Ⅰ, Ⅱ, Ⅲ, Ⅳ) and 3 sets of loading devices were included in this study. The GV-stage oocytes of donkey were cryopreserved by using different solutions and loading devices and were subsequently matured in vitro after thawing. The maturation rate of donkey oocytes was served as an index to compare their performance in cryopreservation. The results demonstrated that the maturation rate of oocytes cryopreserved by solution Ⅱ(28.13%) was significantly higher than that of oocytes cryopreserved by the other 3 solutions(P0.05), but was significantly lower than that of oocytes in control(51.77%,P0.05). The maturation rate of oocytes cryopreserved by OPS(28.13%) was significantly higher than that of oocytes cryopreserved by ordinary glass straws(11.67%,P0.05), but was not significant different compared with that of oocytes cryopreserved by GMP(23.53%,P0.05).
出处 《畜牧与饲料科学》 2016年第3期4-6,10,共4页 Animal Husbandry and Feed Science
基金 国家自然科学基金(31460292)
关键词 玻璃化冷冻 卵母细胞 成熟率 载体 donkey vitrificated cryopreservation oocytes maturation rate loading devices
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参考文献11

  • 1GALLI C,DUCHI R,CROTTI G,et al.Bovine embryo technologies[J].Theriogenology,2003,59(2):599-616.
  • 2RALL W F,FAHY G M.Ice-free cryopreservation of mouse embryos at-196 degrees C by vitrification[J].Nature,1985,313(6003):573-575.
  • 3BEGIN I,BHATIA B,BALDASSARRE H,et al.Cryopreservation of goat oocytes and in vivo derived 2-to 4-cell embryos using the cryoloop(CLV)and solid-surface vitrification(SSV)methods[J].Theriogenology,2003,59(8):1839-1850.
  • 4ALBARRACíN J L,MORATóR,ROJAS C,et al.Effects of vitrification in open pulled straws on the cytology of in vitro matured prepubertal and adult bovine oocytes[J].Theriogenology,2005,63(3):890-901.
  • 5ISACHENKO V,MONTAG M,ISACHENKO E,et al.Aseptic vitrification of human germinal vesicle oocytes using dimethyl sulfoxide as a cryoprotectant[J].Fertil Steril,2006,85(3):741-747.
  • 6周崇,侯云鹏,周光斌,吴通义,金方,洪琼花,朱士恩.小鼠孵化囊胚细管法和OPS法玻璃化冷冻保存技术的研究[J].中国农业大学学报,2004,9(5):26-31. 被引量:5
  • 7李国浩,禹学礼,栗颖华,马永阔,董文听,李振红.封闭式拉长细管冷冻牛卵母细胞的研究[J].畜牧与兽医,2011,43(9):41-44. 被引量:2
  • 8栗颖华,禹学礼,张德勋,陈君怡,许亚坤,李晓霞,昝林森.不同浓度玻璃化冷冻液对牛未成熟卵母细胞发育能力的影响[J].家畜生态学报,2013,34(6):36-39. 被引量:1
  • 9BOS-MIKICH A,WOOD M J,CANDY C J,et al.Cytogenetical analysis and developmental potential of vitrified mouse oocytes[J].Biol Reprod,1995,53(4):780-785.
  • 10VAJTA G,HOLM P,KUWAYAMA M,et al.Open Pulled Straw(OPS)vitrification:a new way to reduce cryoinjuries of bovine ova and embryos[J].Mol Reprod Dev,1998,51(1):53-58.

二级参考文献23

  • 1禹学礼,邓雯,庞有志,王新庄,昝林森,李晓霞,冉林武.开放式拉长细管冷冻法对牛卵母细胞体外受精后发育的影响[J].畜牧兽医学报,2005,36(3):221-224. 被引量:13
  • 2禹学礼,邓雯,庞有志,昝林森.培养条件对牛卵母细胞体外受精后胚胎发育的影响[J].西北农林科技大学学报(自然科学版),2005,33(8):16-20. 被引量:6
  • 3Whittingham D G. Fertilization and development to term of unferti- lized mouse ooeytes previously stored at -196℃C [J]. Reprod Fert, 1977, 49: 89-94.
  • 4Rall W F, Fahy G M. Ice-free cryopreservation of mouse embryos at -196℃ by vitrification [J]. Nature, 1985, 313: 573-575.
  • 5Vajta G, HolmP, Kuwayama M, et al. Open pulled straw (OPS) vitrification : A new way to reduce cryoinjuries of bovine ova and embryos [J]. Molecular Reproduction and Development, 1998, 51: 53-58.
  • 6Yavin S, Aroyo A, Roth Z, et al. Embryo cryopreservation in the presence of low concentration of vitrification solution with sealed pulled straws in liquid nitrogen slush [ J]. Human Reproduction, 2009, 24(4) : 797-804.
  • 7Yu X L, Deng W, Liu F J, et al. Closed pulled straw vitrification of in vitro-produced and in vivo-produced bovine embryos [ J]. Theriogenology, 2010, 73: 474-479.
  • 8Zhou X L, Naib A I, Sun D W, et al. Bovine oocyte vitrification u- sing the Cryotop method: Effect of cumulus cells and vitrification protocol on survival and subsequent development [ J]. Cryobiology, 2010, 61: 66-72.
  • 9Brahmasani S R, Yelisetti U M, Komjeti S, et al. Meiotic maturation of vitrified immature chousingha ( Tetracerus quadricomis) oocytes recovered postmortem [ J]. Cryobiology, 2011,62 : 47-52.
  • 10Arav A, Zeron Y, Leslie S B, et al. Phase transition temperature and chilling sensitivity of bovine oocytes [ J ]. Clyobiology, 1996, 33 : 589-599.

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