摘要
为了阐明水牛半乳糖凝集素3结合蛋白(lectin,galactoside-binding,soluble,3 binding protein,LGALS3BP)基因的结构及功能,本试验采用了3'RACE克隆获得LGALS3BP基因并对其进行了信息学分析。结果表明:水牛LGALS3BP基因编码区长1 668 bp,3'UTR区长590 bp,编码555个氨基酸。BLAST程序比对结果表明克隆获得的LGALS3BP序列与人、非洲象、猫、马、猪、绵羊和牛的同源性分别为82%、82%、84%、83%、94%、94%和97%,系统进化树分析结果表明,LGALS3BP基因在不同物种以及进化的过程中具有高度保守性。蛋白质分析结果表明LGALS3BP蛋白呈弱酸性,第18~19位氨基酸为其蛋白信号肽剪切区域,亚定位于细胞外,存在SR,BACK和BTB等结构域。micro RNA预测显示bta-mi R-2316,bta-mi R-484和btami R-2888等可能为LGALS3BP 3'非翻译区(untranslated region,UTR)潜在靶标。总之,本研究成功克隆获得了水牛LGALS3BP基因并对其进行了系统的信息学分析。
In order to clarify the gene structure and function of LGALS3 BP gene,in this study,buffalo LGALS3 BP gene was cloned by 3＇-Full RACE,analyzed by bio-informatics. The results showed that the coding region of buffalo LGALS3 BP was 1 668 bp,3＇UTR was 590 bp,encoded 555 amino acids. The buffalo LGALS3 BP gene shared 82%,82%,84%,83%,94%,94% and 97% of similar nucleotide sequence with that of Homo sapiens,Loxodonta africana,Equus caballus,Sus scrofa,Ovis aries and Bos taurus respectively. Phylogenetic tree analysis showed that LGALS3 BP gene was highly conserved in different species and evolution. Protein analysis showed LGALS3 BP protein was weakly acidic,its signal peptide was at 18th^19thsides,located in the extracellular,and with the presence of SR,BACK and BTB domain. The prediction of micro RNA/target showed bta-mi R-2316,bta-mi R-484 and bta-mi R-2888 and so on maybe target the 3＇UTR of LGALS3 BP. In a word,buffalo LGALS3 BP gene was successfully cloned and carried out bio-informatics analysis systematicly.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2016年第3期535-541,共7页
Genomics and Applied Biology
基金
农业部转基因重点项目2014ZX0801012B
桂科合14123001-5
水牛基1504005共同资助
关键词
水牛
LGALS3BP
克隆分析
Buffalo
LGALS3BP
Cloning and bio-informatics analysis
