摘要
以一株耐亚硒酸钠的产朊假丝酵母为研究对象,考察了该菌株在不同p H环境下的富硒和谷胱甘肽(GSH)合成能力及抗氧化性能。结果表明,在弱酸胁迫(p H3.5)条件下,富硒产朊假丝酵母胞内有机硒和GSH含量均达到最高水平,分别为1.08 mg/g和18.48 mg/g。通过对酵母胞内γ-谷氨酰半胱氨酸合成酶、过氧化氢酶、超氧化物歧化酶的活性以及丙二醛含量进行测定,发现弱酸胁迫不仅有利于增强GSH的合成能力,也有助于提高酵母细胞的抗氧化能力。该研究结果为发酵法制备高性能富硒产朊假丝酵母提供了可行的技术参考。
The capacities of selenium enrichment and glutathione biosynthesis as well as antioxidant properties of a sodium selenite-resisting Candida utilis Se-1301 were investigated in this study. The results illustrated that the highest intracellular organic selenium and glutathione contents of 1.08 mg/g and 18.48 mg/g,respectively,were obtained under a moderate acid stress environment（p H3.5）. After assaying the activities of intracellularγ-glutamylcysteine synthetase,catalase and superoxide dismutase,together with determining the intracellular malondialdehyde content,the moderate acid stress was found to be not only favored to improve the ability of glutathione biosynthesis,but also helped to increase the antioxidant capacity of the yeast. The results provided a feasible route for efficient preparation of selenium-enriched Candida utilis with high performance.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第8期191-195,共5页
Science and Technology of Food Industry
基金
国家自然科学基金(21506136)
苏州市应用基础研究计划项目(SYN201314)
关键词
富硒酵母
酸胁迫
有机硒含量
谷胱甘肽
产朊假丝酵母
selenium-enriched yeast
acid stress
organic selenium content
glutathione
Candida utilis
