摘要
目的研究异丙酚对早期脂多糖大鼠急性肝损伤的保护作用及机制。方法 2013年3月—2014年10月,选择雄性SD大鼠15只,随机分为正常组(N组)、脂多糖组(L组)、异丙酚+脂多糖组(PL组)各5只。自大鼠尾静脉穿刺固定留置针以便给药,N组大鼠注射生理盐水1 ml;L组大鼠注射脂多糖10 mg/kg;PL组注射异丙酚5 mg/kg+脂多糖10 mg/kg,然后以异丙酚5 mg/(kg·h)持续静脉泵注至1 h。所有大鼠均于给药后1 h处死,采集左心室血行ALT、AST检测;取肝右叶组织检测核因子-κB蛋白表达水平。计量资料采用方差分析,两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果N组、L组、PL组大鼠ALT、AST水平分别为(38.2±19.4)、(101.9±17.2)、(78.3±12.9)、(36.05±16.7)、(104.76±19.4)、(80.0±16.3)U/L,三组比较差异均有统计学意义(均P<0.05)。L组、PL组ALT、AST水平均显著高于N组,PL组低于L组,差异均有统计学意义(均P<0.05)。N组、L组、PL组大鼠核因子-κB蛋白表达水平分别为(0.355±0.080)、(1.227±0.160)、(0.964±0.133),三组比较差异有统计学意义(P<0.05)。L组、PL组核因子-κB蛋白表达水平显著高于N组,PL组低于L组,差异均有统计学意义(均P<0.05)。结论异丙酚对早期脂多糖引起的急性肝损伤有明显的脏器保护作用。核因子-κB蛋白表达的下调可能是异丙酚在早期脓毒症急性肝损伤治疗作用的机理所在。
Objective To observe the protective effect and mechanisms of propofol on acute liver injury in lipopolysaccharide rats during the early phase.Methods March 2013 to October 2014,15 male rats were randomly divided into saline group（N group）,lipopolysaccharide group（L group）,propofol ＋ lipopolysaccharide group（PL group）（n=5）.We established rat model with intravenous injection from tail vein,the rats in N group injected with 1 ml saline;in L group injected with LPS（10 mg/kg）;in PL group injected with propofol（5 mg /kg）and LPS（10 mg/kg）,then continuous intravenous infusion of propofol in 1 h at the speed of 5 mg/（kg·h）.The rats were killed at 1 h after injection.The ALT and AST were test by biochemical detection with left ventricle blood,the nuclear factor κB of hepatic were assessed by immunohistochemical staining protein levels.Measurement data was processed by ANOVA,P〈0.05 was considered statistically significant.Results The level of AST and ALT in N group,L group and PL group were（38.2±19.4）,（101.9±17.2）,（78.3±12.9）,（36.05±16.7）,（104.76±19.4）,（80.0±16.3）U/L,the differences were statistically significant（all P〈0.05）.The levels of AST and ALT in PL group and L group were significantly higher than those in N group,which were lower in PL group than in L group,the differences were statistically significant（all P〈0.05）.The level of nuclear factor- κB protein in N group,L group and PL group were（0.355±0.080）,（1.227±0.160）,（0.964±0.133）,the difference was statistically significant（P〈0.05）.The level of nuclear factor- κB was significantly higher in L group and PL group than that in N group,which was the lowest in L group,and the differences were statistically significant（all P〈0.05）.Conclusion Propofol has obvious protective effects in organ protective effects on acute liver injury at the early stage of lipopolysaccharide rats.The mechanism may be associated with decreasing the level of expression of liver nuclear factor- κB protein.
出处
《社区医学杂志》
2016年第7期36-37,71,共3页
Journal Of Community Medicine
关键词
异丙酚
脂多糖
核因子-ΚB
急性肝损伤
Propofol
Lipopolysaccharide
Nuclear factor-κB
Acute liver injury
