摘要
目的 :分析人非小细胞肺癌(non-small cell carcinoma,NSCLC)组织中c-Myc基因的表达,探讨沉默c-Myc基因在非小细胞肺癌基因治疗中的前景。方法:应用实时荧光定量PCR法检测84例非小细胞肺癌组织及其相应癌旁组织中c-Myc基因mRNA的表达情况。通过脂质体Lipofact AMINE2000将c-Myc基因siRNA瞬时转染至非小细胞肺癌H1299癌株细胞中,顺铂(Cisplatin,cis)药物毒性实验验证c-Myc基因沉默对肿瘤细胞的抑制效果。应用RT-PCR检测H1299细胞中c-Myc基因RNA的表达,CCK-8法检测抑制细胞的效果。结果 :非小细胞肺癌组织中c-Myc基因m RNA的表达水平高于相应的癌旁组织,差异有统计学意义(P<0.05)。c-Myc si RNA瞬时转染后的H1299细胞中,c-Myc基因m RNA的表达水平显著下降(P<0.05)。将c-Myc基因沉默和顺铂联合使用,还可以显著提高顺铂对H1299细胞增殖的抑制效果(P<0.05)。结论 :c-Myc基因在非小细胞肺癌组织中的表达水平明显高于相应癌旁组织,沉默c-Myc基因能显著提高顺铂抑制非小细胞肺癌H1299细胞增殖的作用,c-Myc基因有可能成为NSCLC基因治疗的新靶点。
Objective：To investigate the expression of c-Myc in non-small cell lung cancer（NSCLC）tissues,and to explore the prospects of the c-Myc gene silencing in NSCLC gene therapy. Methods：The expression levels of c-Myc mRNA in 84 NSCLC tissues and the corresponding para-cancerous tissues were detected by RFQ-PCR（real-time fluorescence quantitative-PCR）. C-Myc siRNA was transfected into NSCLC H1299 cells by Lipofect AMINE 2000. Cisplatin toxicity experiments further validated effect of inhibiting tumor cells with c-Myc gene silencing. The expression level of c-Myc m RNA in H1299 cells was detected by RT-PCR,and the effect of inhibiting H1299 cells was detected by CCK-8（Cell Counting Kit-8）. Results：The expression level of c-Myc m RNA in NSCLC tissues was higher than that in the corresponding para-cancerous tissues（P 0.05）. C-Myc si RNA was successfully transfected into NSCLC H1299 cells. After transfection,the expression level of c-Myc mRNA was down-regulated in H1299 cells. Effect of inhibiting tumor cells increased significantly when the c-Myc gene silencing and cisplatin were in combination（P 0.05）. Conclusion：The expression level of c-Myc m RNA in NSCLC tissues is higher than that in the corresponding para-cancerous tissues. The lower expression of c-Myc can significantly inhibit NSCLC H1299 cells. It is suggested that c-Myc may act as an oncogene in NSCLC gene therapy.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第2期151-154,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省六大人才高峰(2012-WS-108)
