摘要
[目的]探究阿片类药物对肝癌患者血清中调节性T细胞表达变化的影响以及小半夏加茯苓汤的干预作用。[方法]收录肝癌患者68例,采用流式细胞仪检测其外周血调节性T细胞的表达水平,并比较使用阿片类药物前后1周T细胞的表达变化;根据患者意愿于阿片类使用1周后开始给予小半夏加茯苓汤干预一周后观察T细胞的表达变化。[结果]肝细胞癌组外周血中辅助性T细胞CD4^+T(CD3^+CD4^+)、抑制性T细胞CD8^+T(CD3^+CD8^+)、调节性T细胞(CD4^+CD25^+)较健康对照组明显减少(P<0.05);肝细胞癌组外周血中NK细胞(CD3-CD16^+CD56^+)较健康对照组明显增加(P<0.05),服用阿片组外周血中CD4^+T、CD8^+T、CD4^+CD25^+较肝癌对照组明显减少(P<0.05);NK细胞较肝癌对照组明显增加(P<0.05);中药干预组明显升高阿片组外周血中CD4^+T、CD8^+T、CD4^+CD25^+,明显降低NK细胞数,差异有统计学意义(P<0.05)[结论]阿片类药物对肝癌患者血清中调节性T细胞功能有明显抑制作用,小半夏加茯苓颗粒能较好的缓解其抑制作用,值得临床推广。
[Objective]To explore the effect of opioid drugs on the expression of regulatory T cells in serum of patients with liver cancer and the intervention effect of xiaobanxia plus fuling Decoction.[Methods]The expression levels of regulatory T cells in peripheral blood were detected by flow cytometry,and the blood was sampled from 68 patients with liver cancer.The expression changes of T cells were compared with those after taking opioids for 1week.After a week of opiate treatment,xiaobanxia plus fuling Decoction intervention was given to patients weeklong according to their wishes,the expression of T cells was then observed.[Results]CD4＋T（CD3＋T＋）,T（CD4＋ ＋）,CD8＋T（CD8＋CD3＋）and T cells（CD4＋CD25＋）were significantly decreased in the peripheral blood of liver cancer group（P〈0.05）,compared with the healthy control group（P〈0.05）;NK cells（CD16CD56＋CD3＋）in peripheral blood of patients with hepatocellular carcinoma were significantly increased（P〈0.05）,CD4＋,CD8＋,CD4＋CD25＋levels in peripheral blood of taking opioid group was found statistical significantly decreased,compared with liver cancer group（P〈0.05）;NK cells than liver cancer group was significantly increased（P〈0.05）;traditional Chinese medicine intervention group significantly increased opioid group of peripheral blood CD4＋T and CD8＋T was significantly reduced NK cell number,the difference was statistically significant（P〈0.05）.[Conclusion]The function of T cells in serum of patients with liver cancer was significantly inhibitedby opioids.The xiaobanxia plus fuling decoction can relieve the inhibitive effect of opioids,and it is worthy of clinical promotion.
出处
《中国中西医结合消化杂志》
CAS
2016年第3期165-167,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
温州市医药卫生科学研究项目(No:2015B72)
关键词
阿片类药物
原发性肝癌
调节性T细胞
中药干预
Opioid drugs
Primary liver cancer
Regulatory T cells
Chinese herbal medicine intervention
