摘要
目的观察补肾益髓(BSYS)方及其拆方补肾(BS)和化痰活血(HTHX)方对实验性自身免疫性脑脊髓炎(EAE)小鼠脑和脊髓中β-淀粉样前体蛋白(β-APP)和微管相关蛋白-2(MAP-2)的作用。方法 56只雌性C57BL/6小鼠随机分为正常对照组(NC)、模型组(MO)、醋酸泼尼松组(PA)、梓醇组(CA)、补肾益髓组(BSYS)、补肾组(BS)和化痰活血组(HTHX),每组8只。对照药给予PA(6 mg/kg),CA(40 mg/kg);BSYS、BS和HTHX组分别以3.02 g,1.44 g和1.57 g生药/kg给药。NC及MO组以等量蒸馏水代替。每日1次,连续灌胃40 d。小鼠发病第25日(急性期)和第40日(缓解期)取脑和脊髓,采用免疫荧光(IF)法检测β-APP表达,免疫组化(IHC)法检测MAP-2的表达。结果发病第25日和第40日,MO组小鼠脑β-APP表达较NC组显著上调(P<0.001),而MAP-2表达则较NC组明显下调(P<0.01或P<0.001),与MO组比较,各治疗组(PA、CA、BSYS、BS和HTHX组)均可明显下调β-APP蛋白表达(P<0.05或P<0.01),上调MAP-2表达(P<0.05或P<0.01)。其中发病第25日小鼠脑β-APP表达PA组效果优于CA、BS及HTHX组(P<0.05)。各组小鼠脊髓内β-APP、MAP-2表达变化趋势与脑相似。结论 BSYS方及其拆方均能减轻轴突损伤及促进其修复,尤以BSYS全方更为显著。
Objective：To observe effects of Bu Shen Yi Sui（BSYS）and its disassembled formulas,Bu Shen（BS）and Hua Tan Huo Xue（HTHX）formulas on the expression of β-amyloid precursor protein（β-APP）and microtubule-associated protein-2（MAP-2）in the brain and spinal cord of mice with experimental autoimmune encephalomyelitis（EAE）.Methods：Female C57BL/6 mice were randomly divided into normal control（NC）,model（MO）,prednisone acetate（PA）,catalpol（CA）,BSYS,BS and HTHX groups.The mice were received daily gavage administration for 40 days.The protein expression of β-APP and MAP-2 in the brains and spinal cords of mice were measured by immunofluorescence（IF）and immunohistochemistry（IHC）respectively on Day 25 and Day 40 after immunization.Results：Compared with MO group,BSYS,BS and HTHX formulas significantly reduced the expression of β-APP in brains and spinal cords of mice（P〈0.05 or P〈0.01）,meanwhile,significantly increased MAP-2（P〈0.05 or P〈0.01）.Conclusion：Both BSYS and its disassembled formulas can relieve axonal injury and promote its repair,especially in the BSYS formula.
出处
《中国中医急症》
2016年第4期565-568,587,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然基金(81273742,81573898)
北京市教委重点项目(KZ201310025023)
北京市属高等学校高层次人才引进与培养计划-长城学者项目(CIT&TCD20140329)
