摘要
目的通过分析辅助生殖技术（ART）男性子代与自然妊娠男性子代脐血染色体核型及Y染色体微缺失,探索ART操作对男性子代染色体核型、Y染色体微缺失的影响。方法选择2008年5月-2012年12月在该院出生的新生儿为研究对象,共74例,根据产妇妊娠方式,分为ART组[体外受精（IVF）36例,卵胞浆内单精子显微注射技术（ICSI）11例]和自然妊娠组（27例）。产妇年龄21～42岁,平均年龄（31±5）岁,ART组产妇平均年龄（32±4）岁,自然妊娠组产妇平均年龄（28±5）岁。穿刺抽取新生儿脐静脉血3～5 ml,进行常规G显带染色法检测染色体核型和提取全血DNA后应用多重PCR技术检测无精子因子（AZF）微缺失,并用SPSS17.0统计软件包进行统计学分析。结果 ART组异常染色体核型9例（19.15%）,自然妊娠组异常染色体核型5例（18.52%）,均为多态性变异,两组间差异无统计学意义（χ^2=0.04,P=0.947）;ART组和自然妊娠组男性子代Y染色体微缺失检测结果均与正常男性阳性对照相同,即两组均未发现Y染色体微缺失。结论与自然妊娠相比,ART操作并未增加男性子代染色体核型异常和多态性变异、Y染色体微缺失的风险,其中ICSI与IVF操作相比,亦未增加男性子代染色体多态性变异、Y染色体微缺失的风险。
Objective To explore the effect of assisted reproductive technology（ ART） on chromosomal karyotypes and Y-chromosome microdeletion of male offsprings by analyzing chromosomal karyotypes and Y-chromosome microdeletion in umbilical cord blood of male offsprings conceived through ART and normal pregnancy. Methods Seventy-four neonates born in the hospital from May 2008 to December2012 were selected and divided into ART group and spontaneous pregnancy group（ 27 cases）,36 cases treated by in vitro fertilization（ IVF）and 11 cases treated by intracytoplasmic sperm injection（ ICSI） were included in ART group. The mothers were aged 21- 42 years old,（ 31±5） years on average,the average age of cases in ART group and spontaneous pregnancy group was（ 32±4） and（ 28±5） years old,respectively. Neonatal umbilical cord blood specimens（ 3-5 ml） were abstracted,routine G banding was used to detect chromosomal karyotypes,whole blood DNA specimens were abstracted to detect azoospermia factor（ AZF） microdeletion by multiplex PCR,the data was analyzed by SPSS 17. 0 software. Results The detection rates of abnormal chromosomal karyotypes in ART group and spontaneous pregnancy group were 19. 15%（ 9 cases） and 18. 52%（ 5 cases）,respectively,and all were polymorphic,there was no statistically significant difference between the two groups（ χ~2= 0. 04,P = 0. 947）; the detection results of Y-chromosome microdeletion among male offsprings in ART group and spontaneous pregnancy group were the same with normal male offsprings,no Y-chromosome microdeletion was detected in the two groups. Conclusion Compared with spontaneous pregnancy,ART does not increase the risk of abnormal chromosomal karyotypes and Ychromosome microdeletion of male offsprings. Compared with IVF,ICSI also does not increase the risk of chromosomal polymorphism and Ychromosome microdeletion of male offsprings.
出处
《中国妇幼保健》
CAS
2016年第8期1683-1686,共4页
Maternal and Child Health Care of China
基金
国家自然科学基金(81170574)
广州市科技计划项目重点项目(11C22120737)
关键词
Y染色体微缺失
染色体核型
染色体多态性
辅助生殖技术
子代
Y-chromosome microdeletion
Chromosomal karyotype
Chromosomal polymorphism
Assisted productive technology
Off spring
