摘要
目的:研究大孔吸附树脂分离纯化短筒兔耳草总黄酮的最佳工艺条件。方法:采用紫外分光光度法和高效液相色谱法分别测定总黄酮质量浓度和木犀草素含量,从而考察8种不同类型的大孔吸附树脂(AB-8,DM130,DM301,S-8,NKA-9,D101,HPD100,H103)的吸附和解吸附性能。结果:AB-8型大孔树脂对短筒兔耳草总黄酮有较好的吸附和洗脱效果,其最佳分离纯化条件:上样液吸附pH值为3.81,上样液质量浓度为0.58 mg·mL^(-1),树脂对上样液的吸附量为66 mL·g^(-1),上样体积流量为1 mL·min^(-1),依次用500 mL水洗脱,50 mL65%乙醇洗脱。经AB-8树脂处理后的总黄酮的质量分数达67.39%、收率为94.73%。结论:AB-8型大孔吸附树脂用于富集纯化短筒兔耳草总黄酮效果较佳,优化后工艺条件稳定可行,适用于短筒兔耳草总黄酮的分离纯化。
OBJECTIVE To investigate purification technology of total flavonoids of Lagotis brevituba Maxim by macroporous resin.METHODS Eight macroporous resins were chosen with static and dynamic adsorption and desorption experiments to optimize purification parameters by using ultraviolet spectrophotometry（UV）and high performance liquid chromatography（HPLC）to determine total flavonoids and luteolin.RESULTS AB-8 macroporous resin was found to have good adsorption and desorption effects.Optimal purification conditions：pH value 3.81,sample mass concentration0.58 mg·mL-1,loaded amount 66 mL·g-1,and loading flow rate 1 mL·min-1.Sample was eluted by 50 mL each of water and 65% ethanol,respectively.Purity of total flavonoids increased to 67.39% after purification,and yield was 94.73%.CONCLUSION AB-8 is an ideal resin with the best enrichment for separating and purifying total flavonoids of Lagotis brevituba Maxim.This optimized process is stable,feasible and suitable for separation and purification of total flavonoids fromLagotis brevituba Maxim.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2016年第7期552-557,共6页
Chinese Journal of Hospital Pharmacy
基金
2012年度兰州市科技局科研项目(2012-1-60)
(2013-3-45)
甘肃省中医药管理局科研项目(编号:GZK-2014-77)
甘肃省自然科学基金项目(编号:1506RJZA042)
关键词
短筒兔耳草
总黄酮
大孔树脂
纯化工艺
Lagotis brevituba Maxim.
total flavonoids
macroporous resin
purification technology
