摘要
建立了一种测定饲料中地克珠利的高效液相色谱-串联质谱方法。以乙腈提取样品中的待测物,正己烷除脂,离心后用高效液相色谱-串联质谱仪,选择多反应监测（MRM）、负离子模式进行定性和定量分析。结果表明,地克珠利在0.5～100 ng/m L浓度范围内线性关系良好,线性相关系数≥0.999,检出限为0.1μg/kg,定量限为0.5μg/kg。地克珠利在0.5、1和5μg/kg三个添加水平下,三种饲料的平均加标回收率在79.2%～94.5%之间,相对偏差在1.7%～14.3%之间。本方法抗干扰能力强,操作简便,灵敏度高,适用于饲料中地克珠利的测定。
An analytical HPLC- MS / MS method was established for determination of diclazuril in feeds. The samples were extracted with acetonitrile,then defatted with n- hexane. The treated samples were analyzed by HPLC- MS / MS after centrifuged. The analysis was performed with Multi Reaction Monitor（ MRM） in negative ion mode. The results indicated good lineartites in the concentration range from 0. 5 to 100 ng / m L for diclazuril,the correlation coefficient was over 0. 999. The limit of detection（ LOD） and the limit of quantitation（ LOQ） were0. 1 and 0. 5 μg / kg,respectively. While the spiked contents of diclazuril standard at 0. 5,1,5 μg / kg,the recoveries and relative standard deviations（ RSD） were in range of 79. 2% ～ 94. 5% and 1. 7% ～ 14. 3%,respectively. With the advantages of good anti- interference ability,rapidness and sensitivity,the method adapted to the determination of diclazuril in feeds.
出处
《中国兽药杂志》
北大核心
2016年第4期57-61,共5页
Chinese Journal of Veterinary Drug
