摘要
以金山绣线菊的茎尖、茎段、叶片、叶柄为外植体,以MS培养基为基本培养基,在初代、继代增殖和生根培养等不同阶段通过添加不同种类和浓度的激素进行对比试验,建立了适宜金山绣线菊组织培养的再生体系。结果表明:诱导愈伤组织培养基为:MS+1.0mg/L 6-BA+0.1mg/L NAA+2.0mg/L 2,4-D,愈伤组织诱导不定芽培养基为:MS+0.8mg/L TDZ+0.10mg/L NAA,生根培养基为:1/2MS+0.25mg/L 6-BA+1.0mg/L NAA,腋芽增殖培养基为:MS+2.0mg/L 6-BA+0.2mg/L NAA。
The stem apex,stem,leaves and petiole of Spiraea×bumalda ‘Golden Mound＇were taken as explants for tissue culture in vitro.Different species and concentrations of hormones were added to MS medium during the different periods of first,subculture and rooting culture for establishing of regeneration system suitable for tissue culture of Spiraea×bumalda‘Golden Mound＇.The results showed that,medium for callus induction was MS＋1.0mg/L 6-BA＋0.1mg/L NAA＋2.0mg/L 2,4-D,medium for adventitious bud induction was MS＋0.8mg/L TDZ＋0.10mg/L NAA,medium for rooting was 1/2MS＋0.25mg/L 6-BA＋1.0mg/L NAA,medium for axillary bud proliferation was MS＋2.0mg/L 6-BA＋0.2mg/L NAA.
出处
《北方园艺》
CAS
北大核心
2016年第8期89-92,共4页
Northern Horticulture
关键词
金山绣线菊
组织培养
再生体系
优化
Spiraea×bumalda‘Golden Mound＇
tissue culture
regeneration system
optimization
