摘要
目的：建立同时测定生脉散中人参皂苷Rb_1、人参皂苷Rc、人参皂苷Rb_2、人参皂苷Rd含量的方法。方法：采用高效液相色谱法。色谱柱为ZORBAX Extend-C_（18）,流动相为乙腈-0.1%甲酸（梯度洗脱）,流速为1.0 ml/min,检测波长为203 nm,柱温为30℃,进样量为10μl。结果：人参皂苷Rb_1、人参皂苷Rc、人参皂苷Rb_2、人参皂苷Rd的检测进样量线性范围分别为0.78~38.75、0.51~25.50、0.43~21.50、0.20~10.00μg（r分别为0.999 6、0.999 6、0.999 7、0.999 7）;精密度、稳定性、重复性试验的RSD≤2%;加样回收率分别为96.6%~101.2%、97.0%~102.1%、99.1%~102.8%、96.3%~101.1%,RSD分别为1.6%、1.7%、1.3%、1.6%（n=6）。结论：该方法简便、快速、稳定性和重复性好、精密度高,可用于生脉散的质量控制。
OBJECTIVE： To establish a method for simultaneous determination for ginsenoside Rbl, ginsenosides Rc, ginsen- osides Rb2 and ginsenosides Rd in Shengmai powder. METHODS：HPLC was performed on the column of Agilent ZORBAX Ex- tend-C18 with mobile phase of acetonitrile -0.1% formic acid（gradient elution） at a flow rate of 1.0 ml/min, wavelength was 203 nm, column temperature was 30 ℃ and the volume injection was 10 ml. RESULTS：The linear was 0.78-38.75 μg for ginsenoside Rbl（r=0.999 6）, 0.51-25.50 μg for ginsenosides Rc （r=0.999 6）,0.43-21.50 μg for ginsenosides Rb2（r=0.999 7） and 0.20-10.00 μg for ginsenosides Rd（r=0.999 7） ; RSDs of precision, stability and reproducibility tests were lower than 2% ; recoveries were 96.6%-101.2%（RSD：1.6% ,n=6）,97.0%-102.1% （RSD= 1.7% ,n=6）,99.1%-102.8% （RSD= 1.3% ,n=6） and 96.3%-101.1% （RSD= 1.6%, n=6）. CONCLUSIONS：The method is simple, rapid with good stability, reproducibility and high precision, and can be used for quality control of Shengmai powder.
出处
《中国药房》
CAS
北大核心
2016年第12期1702-1704,共3页
China Pharmacy
