摘要
目的:探讨加味四君子汤对脾气虚肺癌survivin基因的影响。方法:用苦寒泻下、劳倦、饮食节制复合因素法成功建立小鼠脾气虚证模型后,按实验设计方案处理各组小鼠,同时观察小鼠的整体情况。第17天,摘取肺组织,检测肺组织匀浆上清液中TNF-α。造模后以加味四君子汤灌胃14 d,脱颈椎处死小鼠,剥取肿瘤组织计算瘤重及采用RT-PCR方法检测肿瘤组织中survivin基因表达。结果:脾气虚小鼠的整体状况低下,肺组织匀浆中TNF-α与正常组比较差异有统计学意义(P<0.01)。加味四君子汤可明显改善脾气虚肺癌小鼠的整体情况,升高小鼠脾指数,抑制肺癌生长且可下调脾气虚肺癌组织survivin基因表达。结论:加味四君子汤可以抑制脾气虚证肺癌小鼠肿瘤的生长,其机制可能与下调survivin基因表达有关。
Objective：To discuss the influence of modified Sijunzi decoction on survivin gene in lung cancer mice models with spleen Qi deficiency. Method：Given by multifactor method combined with cold and bitter purgation,fatigue and restricting food intake,the spleen Qi deficiency mice models were successfully established. Lewis cells of lung cancer were inoculated left armpit of mice to construct lung cancer models with spleen Qi deficiency.The mice were administrated with modified Sijunzi decoction or saline solution for 14 days. On the 15 th day,the lung tissue was taken out for testing TNF-α.Then the mice were sacrificed by cervical dislocation,spleen and tumor tissues were taken out. Weighing the spleen and tumor tissues of every mouse,gene expression of survivin was detected by RT-PCR technology. Result：The general condition of mice with spleen Qi deficiency was bad,and TNF-α in the lung tissue homogenate of the model group was significantly lower than that of normal group（P〈0.01）. Modified Sijunzi decoction could obviously improve the general condition of Lewis mice with spleen Qi deficiency,increase systemic immune function,and inhibit the tumor growth. Modified Sijunzi decoction played a role of down-regulating the expression of survivin m RNA. Conclusion：Modified Sijunzi decoction can inhibit growth of lung cancer.Its mechanism may be related to down-regulating level of survivin m RNA.
出处
《山西中医学院学报》
2015年第6期8-10,共3页
Journal of Shanxi College of Traditional Chinese Medicine
基金
浙江省中医药科学研究基金计划(2012ZA032)
关键词
肺癌
脾气虚
加味四君子汤
SURVIVIN
lung cancer
spleen Qi deficiency
modified Sijunzi decoction
survivin
