摘要
目的观察钴、铬离子对小鼠成骨细胞(MC3T3-E1)的细胞毒性及Ⅰ型胶原蛋白合成的影响,探讨钴、铬离子对骨代谢功能的影响机制。方法钴、铬离子分别与小鼠成骨样MC3T3-E1细胞体外培养,噻唑蓝(MTT)法测定细胞活力。ELISA法检测培养上清液中Ⅰ型胶原蛋白含量、RT-PCR检测成骨细胞Ⅰ型胶原蛋白m RNA的表达。结果 MTT结果显示,与对照组相比,钴、铬离子能明显抑制成骨细胞的细胞活力;成骨细胞暴露在钴铬离子下,与对照组相比,24h、48h后:Ⅰ型胶原蛋白的分泌分别下降45.3%、49.2%(P<0.05);Ⅰ型胶原蛋白m RNA表达分别下降26.8%、32.6%(P<0.05)。结论钴、铬离子可抑制成骨细胞Ⅰ型胶原蛋白的分泌及其m RNA的表达,提示其对MC3T3-El细胞可能存在细胞毒性。
Objective To investigate the cytotoxicity of cobalt（Co^2＋） and chromium（Cr^3＋） ions on mice osteoblast（MC3T3-E1）and the effects on the secretion of collagen-Ⅰ from osteoblast under the stimulation of Co^2＋ and Cr^3＋ ions. Methods The osteoblast was cultured in vitro. The cell vitality was assured by MTT test. The ELISA and RT-PCR methods were applied to detect the collagen-Ⅰ on gene and protein levels at 24 and 48 hours after co-culture respectively. Results Compared to the control,MTT test demonstrated that Co^2＋ and Cr^3＋ ions can obviously decrease the cell viability of osteoblast. When the osteoblasts were exposed to Co^2＋ and Cr^3＋ ions for 24 and 48 hours,the releasion of collagen-Ⅰ decreased by 45.3% and 49.2% as compared with the control（P〈0.05）;the expression of collagen-Ⅰ m RNA decreased by 26.8% at 24h,and 32.6% at 48h as compared with the group A（P〈0.05）. Conclusion Co^2＋ and Cr^3＋ ions can inhibit the expression and release of collagen-Ⅰ from osteoblast,and may have a cytotoxic effect on osteoblasts.
出处
《实验与检验医学》
CAS
2016年第2期158-160,共3页
Experimental and Laboratory Medicine
基金
江西省宜春市民生保障科技支撑计划项目(JXYC2013K MA07)
