摘要
[目的]研究龙钻通痹颗粒镇痛、抗炎作用及急性毒性。[方法]①采用扭体、热板法两种镇痛试验方法,每个实验将60只小鼠随机分为生理盐水组,阿司匹林组(50 mg/ml),龙钻通痹组(30 g/kg),每组20只,各组按10 ml/kg剂量灌胃给药,每天1次,连续3 d,然后在预处理后的不同时间分别观察药物对化学、电极热板刺激痛反应的影响。②抗炎试验采用二甲苯致小鼠耳肿胀法。取小鼠40只,随机分为4组,生理盐水组给予等体积蒸馏水,龙钻通痹高、低剂量组按21.4 g/kg和10.7 g/kg剂量给予龙钻通痹颗粒溶液,阿斯匹林组按0.2 g/kg剂量给予阿斯匹林溶液,连续灌胃给药7 d,每天1次。于末次给药后1 h在小鼠右耳涂20μl二甲苯致炎,1 h后处死动物,剪下双耳,用6 mm打孔器切下双耳片称重,以右耳重量减去左耳重,观察其肿胀程度。③急性毒性试验采用小鼠灌胃法,测定龙钻通痹颗粒的最大给药量。[结果]①扭体法试验中,与生理盐水组比较,龙钻通痹组和阿司匹林组均能明显抑制小鼠的扭体次数(P<0.01),延长扭体潜伏期(P<0.01);其中龙钻通痹组镇痛作用与阿司匹林组相似(P>0.05)。表明龙钻通痹颗粒对醋酸引起的疼痛有显著的镇痛作用。②热板法试验中,药后30 min,与同组给药前比较,阿司匹林组能显著延长小鼠的痛阈值(P<0.01);与生理盐水组比较,龙钻通痹组和阿司匹林组均能显著延长小鼠的痛阈值(P<0.05或P<0.01);药后60 min和90min,龙钻通痹组与给药前比较和与生理盐水组同时段比较,均能显著延长小鼠的痛阈值,差异具有显著性意义(P<0.05或P<0.01)。③抗炎试验表明龙钻通痹大剂量组和阿斯匹林组能明显抑制小鼠耳肿胀,具有较好的抗炎作用。④急性毒性试验中,测出龙钻通痹颗粒的最大耐受量为172.8 g/kg,相当于人临床用量的161倍,毒性很小。[结论]龙钻通痹方颗粒具有镇痛、抗炎作用,急性毒性很小。
[Objective]To study the analgesic effect, anti-inflammation and acute toxicity of Longzuantongbi granule.[Methods]①The analgesic experimental methods of body twist method and hot plate test was applied. In each experiment, 60 rats were randomly divided into saline group, aspirin group（50mg/ml）, Longzuantongbi group（30g/kg）, 20 rats for each group. Each group was administered by intragastric gavage following the dose of 10ml/kg, once a day, for 3 days. Then effects of the drug on chemical and electrode-heatplate stimulated-pain response were observed at different time after pretreatment. ②The method of Xylene-induced ear edema in mice was applied on the anti-inflammatory experiment. 40 rats were randomly divided into 4groups. Saline group were given an equal volume of distilled water. High and low dose groups of Longzuantongbi were given Longzuantongbi granule solution following the dose of 21.4g /kg and 10.7g / kg. Aspirin group were given aspirin solution following the dose of 0.2g/kg. They were administered by intragastric gavage for 7 days, once a day. One hour after the last administration, 20μl xylene was scribbled in the right ear of the mice to cause inflammation. The rats were sacrificed one hour later with both ears cut. Both ears were cut with 6mm hole puncher to be weighed. The weight of the left ear was subtracted to the right ear so as to observe the swelling condition. ③ Rats intragastric gavage was applied on acute toxicity experiment to determine the maximum dose of Longzuantongbi granule. [Results]①In body twist experiment, compared with saline group, Longzuantongbi group and aspirin group could inhibit writhing times（P0.01）, and extend writhing incubation period of the mice（P〈0.01）. The analgestic function of Longzuantongbi group was similar to that of aspirin group. It was indicated that Longzuantongbi granule had significant analgestic action on the pain caused by acetic acid. ②In the hot plate test, aspirin group could significantly prolong the pain threshold in mice 30 minutes after administration（P0.01）;Compared with saline group, Longzuantongbi group and aspirin group could significantly prolong the pain threshold in mice 30 minutes after administration（P〈0.05 或 P〈0.01）;Longzuantongbi group could significantly prolong the pain threshold in mice 60 or 90 minutes after administration. Compared with saline group, Longzuantongbi group could significantly prolong the pain threshold at the same time. There was significant difference（P0.05 或P0.01）. ③ Anti- inflammatory experiment showed that high doses of Longzuantongbi group and aspirin group could significantly inhibit mouse ear edema with good anti-inflammatory effect. ④ In acute toxicity experiment, the measure for maximum tolerated dose of Longzuantongbi granule was 172.8g / kg, which was equivalent to 161 times of the human clinical dose with low toxicity.[Conclusion]Longzuantongbi granule has analgesic effect and anti-inflammation with low acute toxicity.
出处
《广西中医药大学学报》
2016年第1期5-8,共4页
Journal of Guangxi University Of Chinese Medicine
基金
国家自然基金项目(编号:81460765)
广西卫生厅中医药管理局课题(编号:GZZJ13-09)
南宁市技术创新项目(南工信科技〔2013〕9号)
关键词
龙钻通痹颗粒
镇痛
抗炎
急性毒性
Longzuantongbi granule
analgesic effect
anti-inflammation
acute toxicity
