摘要
目的探讨人类白细胞抗原（HLA-B）等位基因多态性与江苏地区汉族人群2型糖尿病的相关性。方法采用聚合酶链反应直接测序分型法（PCR-SBT）对118例江苏地区汉族2型糖尿病（T2DM）个体进行HLA-B等位基因分型,计算各等位基因频率和血清型频率,并与中华骨髓库江苏分库汉族志愿者人群（644例和20 248例）进行统计学比较。结果 HLA-B＊1501的等位基因频率在江苏地区汉族T2DM人群中明显升高（8.90%vs 3.03%,Pc=0.000,^OR=3.13,95%CI=1.80～5.42,Power=98.23%）,而HLA-B＊5801的等位基因频率则明显下降（1.69%vs 8.62%,Pc=0.000,^OR=0.18,95%CI=0.07～0.50,Power=91.07%）;相对应地,HLA-B＊15的血清型频率在江苏地区汉族T2DM人群中明显升高（21.18%vs 13.03%,Pc=0.022,^OR=1.79,95%CI=1.26～2.55,Power=90.15%）,而HLA-B＊58的血清型频率则明显下降（1.69%vs 8.62%,Pc=0.000,^OR=0.18,95%CI=0.07～0.50,Power=91.09%）。结论 HLA-B＊1501等位基因可能与江苏地区汉族人群2型糖尿病的易感性有关,而HLA-B＊5801则可能有保护作用。这些结果首次提示HLA-I类基因多态性可能与中国人群2型糖尿病有关。
Objective To explore the association of HLA-B allele polymorphism with type 2 diabetes mellitus（T2DM） in Han ethnicity of Jiangsu region. Methods One hundred and eighteen patients with clinically diagnosed T2 DM were examined. HLA-B typing was performed using polymerase chain reaction with sequence-based genotyping（PCR-SBT）. The frequency of HLA-B alleles and serotypes in the group with T2 DM was compared with that in Han ethnic healthy populations（644 persons and 20248 persons） from Jiangsu branch of Chinese National Marrow Donor Program（CMDP）. Results Compared with the hea lthy donors, there was an increased frequency of HLA-B ＊1501（8.90% vs 3.03%, Pc=0.000,^OR = 3.13, 95% CI = 1.80-5.42, statistical power = 98.23%） and a decreased frequency of HLA-B ＊5801（1.69% vs 8.62%, Pc= 0.000,^OR = 0.18, 95% CI = 0.07-0.50, statistical power = 91.07%） in the group with T2 DM. Accordingly, a higher frequency of HLA-B ＊15 serotype（21.18% vs13.03%,Pc= 0.022,^OR = 1.79, 95% CI = 1.26-2.55, power = 90.15%） and a lower frequency of HLA-B＊58serotype（1.69% vs. 8.62%, Pc= 0.000,^OR = 0.18, 95% CI = 0.07-0.50, power = 91.09%） were found in the T2 DM group. Conclusions HLA-B＊1501 allele may be a marker of susceptibility for T2 DM, in reverse, HLA-B＊5801allele is possibly associated with a resistant effect on T2 DM in Han ethnicity of Jiangsu region. These data suggest,for the first time, the association of HLA class I genes with the T2 DM in Chinese population.
出处
《中国现代医学杂志》
CAS
北大核心
2016年第6期37-44,共8页
China Journal of Modern Medicine
基金
江苏省科技厅科技支撑计划项目资助(No:BE2012739)
