摘要
This study presented an improved CTAB method for extracting DNA from leaves of Populus euphratica Oliv. and Populus pruinosa Schrenk. based on the conventional CTAB method. The results showed that preventing DNA from browning is a key step to obtain the high-quality DNA during DNA extraction, and under the condition of grinding in the presence of liquid nitrogen, adding such three antioxidants as PVP dry powder, Vc and β-mercaptoethanol could prevent DNA from browning effectively. The total DNA extracted by the improved CTAB method was subjected to PCR detection which proved that it totally satisfied the requirements of subsequent study.
该文对常规的CTAB法进行了改良,得到了适合于胡杨、灰叶胡杨硅胶干燥的叶片DNA的提取方法。结果表明DNA提取过程中防止褐化是获得高质量DNA的关键步骤,液氮研磨叶片时加入PVP干粉、抗坏血酸、β-巯基乙醇三种抗氧化剂可有效防止其褐化。此方法获得的总DNA经PCR检测完全满足后续研究的需要。
基金
Supported by Natural Science Foundation of China(31160110)
Open Project of Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin(BRYB1003)
the Principle Fund of Tarim University(TDZKSS201419)~~
