摘要
目的探讨结肠癌转移相关因子-1(MACC1)、间质-上皮细胞转化因子(c-MET)联合癌胚抗原(CEA)在肺腺癌患者并发恶性胸腔积液中的诊断价值。方法将91例胸腔积液患者(良性38例,肺腺癌53例)纳入研究,采用ELISA法测定血清和胸腔积液中MACC1、c-MET浓度,放射免疫法测定CEA浓度。结果肺腺癌患者恶性胸腔积液中MACC1、c-MET和CEA浓度明显高于良性患者胸腔积液中的含量(P<0.05);两组患者血清中MACC1和c-MET浓度无统计学差异(P>0.05)。胸腔积液中MACC1和c-MET含量呈正相关(r=0.728,P<0.01)。根据ROC曲线,以MACC1浓度90.98 pg/ml为临界值,对肺腺癌患者并发恶性胸腔积液的诊断灵敏度为62.26%,特异度为84.21%;c-MET浓度757.67ng/ml为临界值,肺腺癌患者恶性胸腔积液的诊断灵敏度为52.83%,特异度为84.21%。MACC1联合cMET检测灵敏度为75.47%,特异度为92.11%;MACC1、c-MET联合CEA诊断的灵敏度为98.10%,特异度为100%。结论 MACC1和c-MET在肺腺癌患者并发恶性胸腔积液的诊断中具有一定价值,联合CEA检测可明显提高诊断的灵敏度和特异度。
Objective To investigate the diagnostic value of combined measurement of metastasisassociated in colon cancer 1（ MACC1）, carcino embryonie antigen（ CEA） and mesenchymal-epithelial transition factor（ c-MET） in lung adenocarcinoma patients with malignant pleural effusion. Methods Ninetyone patients with pleural effusions were observed,including 53 cases of malignant pleural effusions from lung adenocarcimoma and 38 cases of benign effusions. MACC1 and c-MET levels in plasma and pleural effusion were detected by ELISA. CEA was detected by radioimmunoassay. Results The expressions of MACC1,cMET and CEA in the group of patients with malignant pleural effusion（ MPE） from lung adenocarcinoma were significantly higher than those in the benign group（ P〈0.05）. There was no significant difference of the expression of MACC1 and c-MET in plasma for the two groups（ P〉0. 05）. The expression of MACC1 was correlated significantly positive with the expression c-MET in pleural effusion（ r： 0. 728,P〈0. 01）. A receiver operating characteristic（ ROC） curve analysis was carried out to assess the value of MACC1,c-MET and CEA in MPE from lung adenocarcinoma patients. The cut-of values for MACC1,c-MET and CEA were defined at90.98 pg/ml,757.67 ng/ml and 1.95 ng/ml,respectively. And the sensitivities of MACC1,c-MET and CEA were 62.26%,52.83% and 92.45%,the specificities were 84. 21%,84. 21% and 92. 11%,respectively. The sensitivity and specificity of combined detection of MACC1 and c-MET increased to 75. 47% and 92. 11%,respectively. Moreover,for MACC1,c-MET and CEA,the sensitivity and specificity combined detection increased to 98.10% and 100%,respectively. Conclusions MACC1 and c-MET can be used as the reference indexes for the differentiation of benign pleural effusions and malignant pleural effusions from lung adenocarcinoma patients. Combined examination with CEA can significantly improve the diagnostic value.
出处
《中华肺部疾病杂志（电子版）》
CAS
2016年第2期120-124,共5页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
国家临床重点专科建设项目基金(2012.649)
关键词
肺腺癌
结肠癌转移相关因子-1
间质-上皮细胞转化因子
癌胚抗原
恶性胸腔积液
Lung adenocarcinoma
Metastasis-associated in colon cancer 1
Mesenchymal-epithelial transition factor
Carcino embryonie antigen
Malignant pleural effusion
