摘要
在采用A^2/O工艺处理城市污水的升级改造工程中,于厌氧、缺氧池投加悬浮填料,以提高脱氮除磷效率。经过三年半的稳定运行,显示出良好的改造效果,尤其是脱氮除磷效率得到显著提高。采用Mi Seq Illumina高通量测序法分析该系统的菌群结构,发现在门水平上,厌氧池、缺氧池和好氧池混合液中的优势微生物种群均以变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)为主。其中变形菌门主要分布在β-Proteobacter纲。在厌氧池填料生物膜表面,虽然在门水平上优势微生物菌种也以变形菌门为主,但其主要分布在γ-Proteobacter纲(17.5%)和β-Proteobacter纲(14%)。Proteobacteria和Bacteroidetes的存在可能对整个系统的除磷效果有很大的作用;同时,缺氧池中反硝化菌主要为Thiobacillus、Paracoccus和Pseudomonas。此外,厌氧池填料表面形成了浅红色的生物膜,通过测定其厌氧氨氧化能力发现,NH_3-N和NO_2^--N的去除量之比为1∶2.6。厌氧氨氧化菌在科和目级别,Planctomycetaceae和Planctomycetales均占0.027 8%。除反硝化脱氮外,厌氧池中的厌氧氨氧化现象可能进一步提高了整个系统的脱氮效果。
In the upgrading project using A^2/O process for the treatment of municipal wastewater, the suspended carriers were dosed in the anaerobic and anoxic tanks to improve the nitrogen and phos-phorus removal efficiency. After operating for three and a half years, the whole system showed improved nitrogen and phosphorus removal efficiency. The community structure in the system was analyzed by MiSeq Illumina sequencing technique, and the results showed that the dominant microorganisms in the anaerobic, anoxic and aerobic tanks were Proteobacteria and Bacteroidetes at phylum level and β-Proteobacter at class level. In the biofilm formed on the suspended carrier in the anaerobic tank, the dominant microorganism was Proteobacteria at phylum level, and γ-Proteobacter （17.5%） and β-Proteobacter （14%） at class level. The presence of Proteobacteria and Bacteroidetes might have contributed to the phosphorus removal efficiency. The denitrifying bacteria in the anoxic tank were composed of Thiobacil- lus, Paracoccus and Pseudomonas. The biofilm in the anaerobic tank showed a red color and had an Anammox ability to remove ammonium and nitrite with a ratio of 1 ： 2.6. The Anammox bacteria Planctomyc- etaceae and Planctomycetales at family and order levels both accounted for 0.027 8%. Besides the denitrification efficiency, the Anammox present in the anaerobic tank might have also contributed to the high nitrogen removal efficiency in this system.
出处
《中国给水排水》
CAS
CSCD
北大核心
2016年第9期20-24,29,共6页
China Water & Wastewater
关键词
城市污水
生物脱氮除磷
MBBR工艺
填料
厌氧氨氧化
菌群结构
municipal wastewater
biological removal of nitrogen and phosphorus
moving bed biofilm reactor
suspended carrier
Anammox
community structure
