摘要
目的：建立大鼠脑组织中利培酮含量的测定方法。方法：取大鼠,ig 30 mg/kg的利培酮混悬液,30 min后断头处死,分离脑组织;采用高效液相色谱-紫外法,以盐酸小檗碱为内标,测定大鼠脑组织中利培酮的浓度。色谱条件采用色谱柱Wondasil C18（250 mm×4.6 mm,5μm）;流动相为乙腈-0.01 mol/L醋酸铵溶液（50∶50,V/V）,流速为1.0 ml/min;检测波长为280 nm;柱温为室温;进样量为25μl。结果：利培酮检测质量浓度的线性范围为0.2-27.0μg/ml（r=0.999 9）,方法回收率为97.28%-109.10%,提取回收率为94.59%-97.48%（n=5）,日内和日间RSD均小于5%（n=5）,定量限为0.5μg/ml。大鼠脑组织中利培酮的含量为（3.48±0.50）μg/g,RSD为14.7%（n=6）。结论：本方法简便、快速、灵敏、准确度高,可用于大鼠脑组织中利培酮的含量测定。
OBJECTIVE：To establish the method for the content determination of risperidone（RIS）in cerebral tissues of rats.METHODS：Rats were given RIS suspension 30 mg/kg,ig,and sacrificed 30 min later to separate cerebral tissue. The concentration of RIS in cerebral tissues of rats was determined by HPLC system with UV detector using berberine hydrochloride as internal standard. The chromatographic conditions were that Wondasil C18column（250 mm×4.6 mm,5 μm）was used with mobile phase consisted of acetonitrile-0.01 mol/L ammonium acetate（50 ∶ 50,V/V）at the flow rate of 1.0 ml/min;the detection wavelength was set at 280 nm;the column temperature was maintained at room temperature,and sample size was 25 μl. RESULTS：There was a good linear relationship of RIS concentration in cerebral tissues of rats within the range of 0.2-27.0 μg/ml（r=0.999 9）. The method recovery was from 97.28% to 109.10% and extraction recovery was from 94.59% to 97.48%（n=5）;intra-day and inter-day RSDs were both below 5%（n=5）. The limit of quantitation was 0.5 μg/ml. The content of RIS in cerebral tissue of rats was（3.48 ±0.50）μg/g（RSD=14.7%,n=6）. CONCLUSIONS：The established method is simple,rapid,sensitive and accurate for the content determination of RIS in cerebral tissues of rats.
出处
《中国药房》
CAS
北大核心
2016年第13期1782-1784,共3页
China Pharmacy
基金
内蒙古自治区自然科学基金项目[No.2015MS(LH)0801)]
内蒙古医科大学科技百万工程项目(No.YKD2014KJBW012)
