摘要
背景:生物玻璃与磷酸钙骨水泥复合形成的可吸收生物玻璃注射体,能在材料界面与人体骨组织之间形成牢固化学键,提高移植物与界面的稳定性。目的:观察可吸收生物玻璃注射体在骨质疏松大鼠模型椎体内的支撑及诱导成骨机制。方法:切除30只雌性SD大鼠双侧卵巢,制备骨质疏松模型。造模成功后,随机分成3组,在L_(3-5)椎体骨缺损处分别置入聚甲基丙烯酸甲酯、注射性磷酸钙骨水泥和可吸收生物玻璃注射体。置入后12周取置入节段椎体,进行生物力学、降解性能、Ca、P、碱性磷酸酶、骨密度、骨形态发生蛋白2和转化生长因子β水平检测,以及骨组织切片观察。结果与结论:可吸收生物玻璃注射体组的降解性能、抗压强度、表面羟基磷灰石沉积量、骨密度显著高于其余两组(P<0.05),骨小梁相对体积、厚度和数量显著大于其他两组(P<0.05),血清Ca、碱性磷酸酶、骨形态发生蛋白2和转化生长因子β水平显著高于其他两组(P<0.05),血清P水平低于其他两组(P<0.05)。结果表明,可吸收生物玻璃注射体可增强骨质疏松椎体内支撑力,诱导成骨,可能与提高骨形态发生蛋白和转化生长因子β的表达有关。
BACKGROUND： Absorbable bio-glass injection composed by bio-glass and calcium phosphate bone cement can be tightly combined with bone tissue by a strong chemical bond to improve the stability of the bone-implant interface. OBJECTIVE： To investigate the effect of absorbable bio-glass injection to support the vertebral body of osteoporosis mice and its mechanism of osteogenic induction. METHODS： Osteoporosis models were prepared in 30 female Sprague-Dawley rats undergoing bilateral ovariectomy. After modeling, model rats were randomly divided into three groups, and given polymethylmethacrylate, injectable calcium phosphate bone cement and absorbable bio-glass injection into L_（3-5） vertebral bone defects, respectively. L3-5 segments were removed at 12 weeks after implantation to detect the biomechanical and degradation properties, levels of calcium and phosphate, alkaline phosphatase activity, bone mineral density, levels of bone morphogenetic protein 2 and transforming growth factor β, as well as histological observation. RESULTS AND CONCLUSION： In the absorbable bio-glass injection group, the degradation properties, compressive strength, surface hydroxyapatite deposition amount and bone mineral density were significantly higher than those in the other two groups（P〈0.05）; trabecular bone relative volume, thickness and number were significant higher than those in the other two groups（P〈0.05）; serum levels of calcium, alkaline phosphatase, bone morphogenetic protein 2 and transforming growth factor β were significantly higher than those in the other two groups（P〈0.05）; but the level of serum phosphate was lower than that in the other two groups（P〈0.05）. These results show that the absorbable bio-glass injection can enhance the support for the osteoporotic vertebral body and induce osteogenesis, probably by increasing bone morphogenetic protein 2 and transforming growth factor β levels.
出处
《中国组织工程研究》
CAS
北大核心
2016年第16期2310-2316,共7页
Chinese Journal of Tissue Engineering Research
基金
湖北省卫生厅青年人才项目(WJ2015Q040):骨癌痛大鼠痛觉调控核团特异性基因与蛋白表达谱研究~~
