摘要
目的:观察糖尿病大鼠肾组织中4羟基壬烯醛(4-HNE)的表达,并探讨促红细胞生成素(EPO)的肾脏保护作用机制。方法:链脲佐菌素(STZ)腹腔注射构建糖尿病大鼠模型,随机分为糖尿病组(D组),EPO组(E组),并设正常对照组(C组)。E组给予EPO皮下注射。分别检测各组大鼠24 h尿蛋白量、血尿素氮(BUN)及血肌酐(Scr)。免疫组化及Western-blot观察各组大鼠肾组织4-HNE的表达情况。结果:E组及D组4周、8周及12周肾脏中的4-HNE的表达较C组明显升高,免疫组化染色积分各组差别有统计学意义(P<0.05),E组4-HNE的表达量低于同时间的D组,差别有统计学意义(P<0.05),Western-blot结果与免疫组化结果相符合。结论:糖尿病大鼠肾脏4-HNE升高,EPO可通过抑制脂质过氧化降低4-HNE的表达,从而起到肾脏保护作用。
Objective： To observe the expression of 4- hydroxynonenal（ 4- HNE） in the kidney of STZ induced diabetic rats and investigate the protective effect of erythropoietin（ EPO）. Methods： Rats were randomly divided into control group（ group C）,diabetic group（ group D） and EPO treated group（ group E）. Rats of group D and group E were given STZ by intraperitoneal injection to establish models of diabetes. EPO was administered to the diabetic rats in group E for 12 weeks. 24- hour urinary protein,BUN and Scr of each group were measured at the 4th,8th and 12 th weeks. The immunohistochemistry and Western- blot were used to detect the expression of 4- HNE in renal tissue. Results： The expression of 4- HNE in group E was significantly lower than group D（ P〈0. 05）,but was much higher than group C（ P〈0. 05）. Conclusion： As an indicator of lipid peroxidation,the expression of4- HNE significantly increases in the kidney of STZ induced diabetic rats. EPO may protect the diabetic kidney through inhibiting the level of lipid peroxidation and decreasing the expression of 4- HNE.
出处
《中国中西医结合肾病杂志》
2016年第3期207-210,I0001,I0002,共6页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
青岛市科技局基金资助项目(No.13-1-3-46-nsh)
青岛市医疗卫生优秀人才培养项目(No.青卫科教字[2014]8号)
