摘要
目的:研究Graves病患者外周血中Th17细胞及IL-17、IL-23的表达水平并探讨其临床意义。方法:用流式细胞仪检测29例Graves病患者治疗前后(GD组,GD缓解组)和29名健康对照者(NC组)外周血PBMC中CD4^+IL-17^+T细胞比例,用ELISA法检测血清中IL-17和IL-23浓度,用电化学发光法检测血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、促甲状腺激素(TSH)水平,用放射受体分析法测定抗促甲状腺激素受体抗体(TrAb)水平。结果:3组之间年龄、性别差异均无统计学意义(F=0.007 5,P>0.05;χ~2=0.4213,P>0.05)。GD组和GD缓解组Th17比例、IL-17、IL-23的水平显著高于NC组,差异有统计学意义(P均<0.05)。GD缓解组的Th17比例、IL-23浓度显著低于GD组,但GD缓解组IL-17浓度与GD组的差异无统计学意义(P>0.05)。Th17比例、IL-17、IL-23与FT3、FT4、TrAb均呈正相关(r值依次为:0.588 2、0.3372、0.371 0;0.549 6、0.287 5、0.342 7;0.361 0、0.420 8、0.330 8;P均<0.05)。Th17比例、IL-17、IL-23与TSH均呈负相关(r值依次为-0.319 7、-0.472 8、-0.428 2;P均<0.05)。结论:Th17细胞及其相关细胞因子IL-17、IL-23在GD患者外周血中均高表达;并与GD患者的甲功4项指标具有不同程度的相关性。Th17细胞及相关细胞因子IL-17、IL-23能够作为GD的生物学标记物。
Objective： To explore the proportions of Th17 cells in the peripheral blood and levels of IL-17,IL-23 in the serum of patients with Graves＇ disease（ GD） and their clinical significance. Methods： We studied 29 patients with GD（ GD group）,and reevaluated the GD group after therapy（ euthyroid GD group）. 29 gender-and age-matched volunteers were selected as the normal control（ NC group）. The proportions of Th17 cells were investigated by flow cytometry. The levels of IL-23,IL-17 in the serums were measured by ELISA. The levels of FT3,FT4,TSH were determined by ECLIA and the levels of Tr Ab were tested by RRA. Results： There were no significant difference among 3 study groups in sex and age match（ F = 0. 0075,P〉0. 05; χ^2= 0. 4213,P〉0. 05）. The proportions of Th17 cells and the levels of IL-17,IL-23 were increased in the GD and euthyroid GD patients compared with the control group（ respectively,P〈0. 05）. The proportions of Th17 cells and the concentrations of IL-23 in euthyroid GD group were significantly lower than those of GD group（ respectively,P〈0. 05）,but there were no significance in the concentrations of IL-17 between euthyroid GD group and GD group（ P〉0. 05）. Correlation analysis revealed that the proportions of Th17 cells,and the levels of IL-17,IL-23 were positively correlated with the levels of FT3,FT4,Tr Ab（ r = 0. 588 2,0. 337 2,0. 371 0; 0. 549 6,0. 287 5,0. 342 7; 0. 361 0,0. 420 8,0. 330 8; P〈0. 05,for all parameters）,and were negatively correlated with the levels of TSH（ r =- 0. 319 7,- 0. 472 8,- 0. 428 2; P〈0. 05,for all parameters）. Conclusion： Th17 cells and their related cytokines IL-17,IL-23 are highly expressed in the serum of patients with GD. Th17 cells and their relative cytokines have certain relevance with 4 thyroid function parameters of the patients with GD,which can be used as biological markers for GD.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第4期563-566,共4页
Chinese Journal of Immunology
