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腺病毒载体介导的H9C2心肌细胞外源基因表达系统的构建

Construction of Exogenous Gene Expression System in H9C2 Myocardial Cell Mediated by Adenoviral Vector
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摘要 本研究旨在探讨不同剂量的脂质体与腺病毒载体对转染H9C2细胞的效率的影响。应用常规剂量脂质体与减半剂量脂质体介导梯度剂量腺病毒载体转染H9C2细胞,并通过观察报告基因GFP表达计算各转染条件下的转染效率。结果发现应用常规剂量减半的脂质体反而能获得更高的转染效率;腺病毒载体与转染效率具有明显的量效关系,但是当载体量过大时转染效率有所下降。因此脂质体与腺病毒载体都具有一定的细胞毒性,细胞处于良好的状态可能更有利于外源基因的转入。 To investigate and compare the transfection efficiency into H9C2 myocardial cells by adenoviral vectors with different dose of liposomes. H9C2 cells were transfected by different dose of adenoviral vectors with the routine and half dose of liposomes, and the transfection efficiency was determined based on the expression of GFP gene. As a result, the transfection efficiency with half dose of liposomes was more than that with routine dose,and there was a significant dose-response relationship between adenoviral vectors and transfection efficiency;however, when the vector amount was too high, the transfection efficiency decreased. In conclusion, both liposomes and adenoviral vectors have some cytotoxicity, and the cells in good condition were easily transfected.
作者 何原 陈涛 严国森 雷倩 张宇 成力 黄石安 陈灿 李果明 雷桅
机构地区 广东医科大学心血管疾病研究室 广东医科大学附属医院心血管内科中心
出处 《基因组学与应用生物学》 CAS CSCD 北大核心 2016年第4期752-755,共4页 Genomics and Applied Biology
基金 国家自然科学基金项目(81300035 81270212) 广东省自然科学基金项目(S2013040012115)共同资助
关键词 脂质体 腺病毒载体 转染 H9C2心肌细胞 基因 Liposome Adenoviral vector Transfection H9C2 myocardial cells Gene
分类号 R54 [医药卫生—心血管疾病]
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参考文献16

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基因组学与应用生物学
2016年 第4期

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