摘要
目的观察盆炎方对盆腔炎性疾病后遗症大鼠子宫组织中肿瘤坏死因子-α(TNF-α)、表皮生长因子(EGF)蛋白表达的影响。方法以"机械性损伤+混合菌种接种法"建立盆腔炎性疾病后遗症SD雌性大鼠子宫模型。造模成功后随机分为模型对照组、妇科千金胶囊组及盆炎方高、中、低剂量组,另设空白对照组和假手术组共计7组,每组8只。分别以盆炎方高、中、低剂量药液、妇科千金混悬液及同等体积蒸馏水灌胃21 d。采用免疫组化法检测各组大鼠子宫组织中TNF-α、EGF蛋白表达的差异。结果与模型组比较,盆炎方各剂量组大鼠子宫组织的TNF-α表达明显降低(P<0.05);盆炎方中、高剂量组大鼠子宫组织的EGF蛋白表达明显升高(P<0.05);结论盆炎方高剂量组能够显著降低盆腔炎性疾病后遗症大鼠子宫中TNF-α的表达,明显增强盆腔炎性疾病后遗症大鼠子宫中EGF的表达,盆炎方对盆腔炎性疾病后遗症有积极治疗作用。
Objective To observe the influence of Penyan decoction（PYD） on the TNF-α and EGF expression of uterus in rats with pelvic inflammatory disease sequelae. Methods The uterus model rats with pelvic inflammatory disease sequelae were established by using ＂the mechanic injury and mixed bacteria inoculation＂ method. The successful model rats were randomly divided into model group, Fuke Qianjin capsule group, and high, medium and low dose of PYD groups and PYD low dose group, rats were also assigned into sham-operation group and normal control group, 8 rats in each group. The rats were intragastric administration with high, medium, low dose PYD liquid, Fufang Qianjin capsule and the same volume of distilled water for 21 days, respectively. The TNF-α and EGF expression of rats in all groups were detected by using the immunohisto chemical method. Results Compared with the model group, the expression of TNF-α was significantly decreased in the rats with different dose groups（P〈0.05）; the expression of EGF in medium dose group and high dose group was significantly higher（P〈0.05）. Conclusion PYF group with high dose could significantly reduce the expression of TNF-α and increase the expression of EGF in uterus of rats with pelvic inflammatory disease sequelae, and the PYD show positive treatment effect on sequelae of pelvic inflammatory disease.
出处
《湖南中医药大学学报》
CAS
2016年第4期15-18,共4页
Journal of Hunan University of Chinese Medicine
基金
谢剑南名老中医传承工作室资助项目
