摘要
目的:探索结直肠癌患者手术前后肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)表达水平的变化及其临床意义。方法:对75例试验组结直肠癌患者和对照组43例健康体检者分别采用硝酸还原法及双抗夹心ELISA法检测手术前及手术7d后NO及TNF-α、IL-1β、IL-6表达水平,比较2组结果变化,同时对手术前后的检测指标作相关性分析。结果:结直肠癌患者手术前血清中TNF-α、IL-1β、IL-6浓度明显高于正常对照组(P<0.05),而NO浓度明显低于正常对照组(P<0.05),手术治疗后7d,TNF-α、IL-1β、IL-6血清含量明显降低(P<0.05),而NO含量恢复正常水平。手术前后TNF-α与IL-6、NO、IL-1β及NO与IL-6、IL-1β均无相关性,而IL-1β与IL-6之间呈明显的正相关。结论:血清中TNF-α、NO、IL-1β、IL-6浓度与结直肠癌密切相关,可作为结直肠癌诊断及手术治疗新的参考指标。
Objective：To explore the pre and post-operative changes and its clinical significance of the levels of TNF-α,NO,IL-1β and IL-6in the patients with colorectal cancer.Method：The levels of NO and TNF-α,IL-1β,IL-6 in 75 colorectal cancer patients and control group were detected by nitrate reduction and double-antibody sandwich ELISA,and the results were compared.Result：In preoperative colorectal cancer patients,the levels of serum TNF-α,IL-1β,IL-6 were significantly higher than those in controls（P〈0.05）,while the NO level was significantly lower（P〈0.05）.Seven days after operation,the levels of TNF-α,IL-1β,IL-6 were lower than those before（P〈0.05）,and the NO level returned to normal.Conclusion：TNF-α,NO,IL-1β and IL-6 might play important roles in colorectal cancer,and they may provide new reference for colorectal cancer early diagnosis.
出处
《临床血液学杂志（输血与检验）》
CAS
2016年第2期283-285,共3页
Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)
