摘要
通过构建拟南芥WHIRLY2基因超表达植株和鉴定这个基因的突变体,对WHIRLY2基因调控拟南芥角果的发育过程进行了分析.表型观察结果显示过量表达WHIRLY2基因的植株角果发育异常,出现变黄、干瘪和早衰现象,角果果皮细胞中叶绿体的数量明显比野生型少,叶绿体中淀粉粒的数目比野生型明显增多.进一步对角果发育、能量代谢、线粒体基因组稳定、叶绿体淀粉粒代谢相关基因的表达丰度进行qRT-PCR分析,发现WHIRLY2超表达植株角果中与线粒体能量代谢相关的nad1、cytc382、rad50和同时作用于线粒体和叶绿体的atp9基因的表达发生变化.通过线粒体基因组的结构分析和蛋白质凝胶迁移试验,发现WHIRLY2蛋白可以结合在线粒体基因组的nad1和cytc382基因启动子的4个端粒重复序列AAAATCCCC上,推测WHIRLY2可能通过调控nad1和cytc382基因的转录从而参与角果发育的调控.
To elucidate the role of WHIRLY2 in mitochondrial activity, overexpressing and knockout lines of Arabidopsis thaliaua Co-lumbia were cultured. Phenotype analysis showed that silique of WHIRLY2-overexpressing line became dry, yellow and showed earlysenescence. Decreasing chloroplast number and increasing starch granule number were detected in pericarp cell of WHIRLY2 mutantscompared to wild-type plant. Subsequent quantitative RT-PCR were conducted to analyze expression levels of genes related to siliquedevelopment, energy metabolism, stability of mitochondrial genome and chloroplast differentiation in WHIRLY2 mutants. Resultsshowed that transcription levels of genes involved in mitochondrial energy metabolism, including rad1, cytc382, tad50 and atp9,were increased in the silique of WHIRLY2- overexpressing lines. Mitochondrial genome structure and electrophoretic mobility shift as-says revealed that WHIRLY2 protein was able to bind to the 4xTelomere repeats of promoter region of rad1 and cytc382 genes. Itsuggested that WHIRLY2 may influence mitochondrial activity via gene expression of rad1 and cytc382 to regulate silique develop-ment in Arabidopsis.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2016年第3期277-281,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家自然科学基金资助项目(31470383
31400260)
福建省"百人计划"基金资助项目(KXR14002A)
