摘要
目的:探讨复方番石榴制剂对2型糖尿病胰岛素抵抗大鼠脂肪代谢及胰腺组织病理形态的影响。方法:给高热量饲料喂养8周后的大鼠腹腔注射小剂量链脲佐菌素溶液,复制2型糖尿病动物模型,随机分为模型组、二甲双胍组、复方番石榴制剂高剂量组和低剂量组。另设正常组,饲以普通饲料。灌胃给药6周后处死大鼠,末次给药后禁食12 h,检测血脂及脂蛋白指标,并摘取胰腺组织观察组织病理形态。结果:与模型组比较,各治疗组可不同程度地降低胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)和游离脂肪酸(FFA),显著升高高密度脂蛋白胆固醇(HDL-C)(P<0.05),其中,复方番石榴制剂高剂量组和二甲双胍组有明显的降脂作用。光镜下观察胰腺组织病理形态,可见模型组损伤最明显,复方番石榴制剂组及二甲双胍组均呈现不同程度的损伤。结论:复方番石榴制剂有明显的改善脂代谢紊乱及减轻胰腺病理损伤的作用。
Objective：To study the effects of compound preparation of Fanshiliu on lipid metabolism and pancreatic pathological changes in type 2 diabetes mellitus（T2DM） rats with insulin resistance.Methods：After fed with high-calorie food for 8 weeks,the rats received intraperitoneal injection with low dose of streptozotocin（STZ） to induce T2 DM animal model,and then the rats were randomly divided into the model group,metformin group,high and low dose group of compound preparation of Fanshiliu.The normal group was given ordinary feed.The rats were sacrified after intragastric administration for six weeks,and fasted for 12 hours after the last administration.The lipid and lipoprotein indices were detected,and pathological morphology of pancreatic tissue was observed.Results：Compared with those in the model group,TC,TG,LDL-C and FFA in each treatment group were reduced,and HDL-C was increased（P〈0.05）.Fanshiliu high dose group and metformin group had obvious hypoglycemic and lipid-lowering effects.The results of pathological morphology of pancreatic tissue under a light microscope showed that the damage was the most obvious in the model group.Compound preparation of Fanshiliu groups and metformin group showed damage in varying degrees.Conclusion：Fanshiliu preparation can effectively adjust blood lipid levels,enhance the insulin sensitivity and reduce damage in pancreas in T2 DM rats.
出处
《中国药师》
CAS
2016年第5期866-869,共4页
China Pharmacist
基金
广东省中医药局立项资助项目(编号:20141282)
中山市科技局重点资助项目(编号:2014A1FC004)
关键词
2型糖尿病
复方番石榴制剂
胰岛素抵抗
脂代谢
胰腺组织病理形态
Type 2 diabetes
Compound preparation of Fanshiliu
Insulin resistance
Lipid metabolism
Pathological morphology of pancreatic tissue
