摘要
在植物病害研究中,观察寄主植物被病原菌入侵的过程非常重要。Solophenyl Flavine 7GFE染料可附着于菌丝,在波长为330~380 nm的激发光下被激发出蓝色荧光。为了更好的观察寄主植物中病原真菌的侵染情况,本实验以Solophenyl Flavine 7GFE为染剂,对寄主植物中病原真菌的侵染情况进行了观察研究。结果显示,用0.002%（M/V）Solophenyl Flavine 7GFE溶于0.1 mol/L Tris-HCl（p H 8.5）配制的染色液染色5 min的效果最佳;使用95%乙醇溶液替代0.15%三氯乙酸（W/V）酒精溶液∶氯仿（V/V）（4∶1）对寄主植物叶片脱色的方法操作简便、毒害较低;染色时省略了番红预染步骤。将改良的染色方法用于晚疫病菌入侵的马铃薯叶片观察取得了良好的效果。该技术是一种改良的、快速有效、安全无毒的观察真菌菌丝入侵植物的荧光染色方法,也适用于观察其他真菌入侵寄主植物组织的过程。
Research on host plants invaded by pathogenic fungi have great significance in plant disease. Solophenyl flavine 7GFE can attach to hyphae,which give fluorescence under 330-380 nm light. This paper modified a fluorescence staining method using solophenyl flavine7 GFE to observe fungal hyphae invading plants. Results showed that the most suitable leaf staining condition was 0. 002%（ M / V） solophenyl flavine 7GFE dissolved in 0. 1 mol / L Tris-HCl（ p H 8.5） for 5 min. Using 95% ethanol instead of 0.15% trichloroacetic acid（ W/V） alcohol solution ∶ chloroform（ V / V） 4 ∶ 1 for decolorization and omission of safranine staining simplified operation and reduced toxicity. The modified staining method was used to observe potatoleaves invaded by Phytophthora infestans and achieved good effects. The modified staining method was fast,effective and non-toxic,and was suitable for observation of the invasion process of fungal pathogens and hosts.
出处
《植物科学学报》
CAS
CSCD
北大核心
2016年第2期316-324,共9页
Plant Science Journal
基金
国家自然科学基金项目(31201502)
高校博士学科点专项科研基金项目(20124320120015)~~
