摘要
目的：研究与比较青翘和老翘中的挥发性成分。方法：采用水蒸气蒸馏法提取挥发油。采用气相-质谱联用技术测定药材中挥发油成分的含量。色谱条件：色谱柱为HP-5MS,进样口温度为200℃,载气为氮气,分流比为20∶1,程序升温,流速为1 ml/min,进样量为1μl;质谱条件：离子源为电轰击离子源,电子能量为70 e V,接口温度为280℃,离子源温度为230℃,四极杆温度为150℃,电子倍增电压为2 300 V,质量扫描范围为29~350 amu。结果：挥发油提取得率高低顺序为青翘心〉青翘〉青翘壳〉老翘;青翘、老翘挥发油有18个共有峰,均以β-蒎烯含量最高,其次为α-蒎烯、β-水芹烯、松油烯-4-醇、松油烯等;此外,4-亚甲基-1-环己酮、3-环己烯-1-甲醇、对异丙基苯甲醛、香树烯、棕榈酸、肉豆蔻醛6种成分仅老翘挥发油中可检出;而α-水芹烯、罗勒烯、α-松油醇、α-松香芹酮、Z-薄荷醇、乙酸龙脑酯、α-库比烯、大根香叶烯D 8种成分仅在青翘挥发油中检出。结论：青翘与老翘挥发油的主要化学成分基本一致,但也存在一定差异,该发现或许能够为连翘药效差异物质基础的研究提供一定依据。
OBJECTIVE：To study and compare the volatile constituents in Forsythia suspensa and old F. suspensa. METHODS：Steam distillation method was adopted to extract volatile oil. GC-MS was adopted to determine the content of volatile oil：the chromatographic conditions：the column was HP-5MS,inlet temperature was 200 ℃,the carrier gas was nitrogen,split ratio was 20 to 1,by programmed temperature,flow rate was 1 ml/min,the injection volume was 1 μl;MS conditions：ion source was electric bombardment ionization source,electron energy was 70 e V,the interface temperature was 280 ℃,ion source temperature was 230 ℃,quadrupole temperature was 150 ℃,electron multiplier voltage was 2 300 V,and mass scanning range was 29-350 amu. RESULTS：The extraction yield order of volatile oil was that F. suspensa heart was higher than F. suspensa,followed by F.suspensa shell and old F. suspensa;there were 18 common peaks in the volatile oil,and the content of β-pinene was the maximum,followed by α-pinene,β-phellandrene,terpinen-4-ol and terpinene;in addition,4-methylene-1-cyclohexanone,3-cyclohexene-1-methanol,cumene formaldehyde,1,7-trimethyl-4-methylenedecahydro,palmitic acid,nutmeg aldehyde can only be detected in the volatile oil of old F. suspensa;while α-phellandrene,ocimene,α-terpineol,α-pinocarvone,cis-piperitol,bornyl acetate,α-cubebene and germacrene D can only present in F. suspensa. CONCLUSIONS：The main chemical components in the volatile oil of F. suspensa and old F. suspensa are basically the same,but there are also some differences. The discovery can provide some basis for the material basic research in the pharmacodynamic differences of F. suspensa.
出处
《中国药房》
CAS
北大核心
2016年第15期2087-2090,共4页
China Pharmacy
基金
国家自然科学基金资助面上项目(No.81470169)
关键词
青翘
老翘
挥发油
气相色谱-质谱联用技术
成分分析
Forsythia suspensa
Old Forsythia suspensa
Volatile oil
GC-MS
Constitution analysis
