摘要
为了探索重金属锌长期胁迫对麦长管蚜(Sitobion avenae)取食行为的变化影响,在模拟自然的实验室条件下,用不同浓度Zn^(2+)溶液浇灌土壤,通过土壤-小麦-蚜虫体系连续处理麦长管蚜15代,用EPG(刺探电位技术)对第1、5、10、15代成蚜的取食行为进行了监测。结果表明,第1代和第5代时,200 mg/kg的Zn^(2+)处理后np波和C波的总持续时间和数量显著低于对照,800mg/kg的Zn^(2+)使其显著增加。到第15代,高剂量的Zn^(2+)处理后np波和C波的总持续时间和数量均显著高于对照。涉及分泌唾液的E1波持续时间及涉及被动取食营养的E2波出现次数并未受到低剂量Zn^(2+)的显著影响,但高剂量的Zn^(2+)处理后单独E1波、伴随稳定E2的E1波总持续时间及E2波的数量均显著降低。麦长管蚜的取食行为会受到重金属锌的影响并且会在高剂量Zn^(2+)的胁迫条件下产生积累效应,而低剂量的Zn^(2+)则促进麦长管蚜对小麦的取食行为。针对重金属而言,此效应发生改变的关键浓度为400 mg/kg,蚜虫取食行为发生改变的关键世代为第5代和第10代。
The aim of current study was to determine the effects of long-time exposure of Zn^2＋ on feeding behavior of English green aphid, Sitobion avenae （Fabricius）. We had stimulated the natural Zn^2＋ stress by watering the different concentration of Zn^2＋ solutions to the wheat plants under controlled greenhouse conditions. The 1^st, 5^th, 10^th and 15^th generations of apterous S. avenae populations reared on those plants were used in the experiments. At the three leaves stage （ about 14 days after germination）, a single apterous adult S. avenae was transferred to the plants and monitored by electrical penetration graph （EPG）. The results showed that the feeding behavior of S. avenae was significantly influenced by Zn^2＋ stress. In particular, at the 1 ^st and 5^th generations, total duration and number of np and C waveforms were lower than the control at the concentration of 200 mg/kg, while they were higher than the control at 800 mg/kg. Compared with the control, at 15^th generation, these parameters of S. avenae under the high dose were significantly increased. Moreover, for the penetrating feeding on phloem, no significant influence was found about the duration of E1 involved in salivation and number of E2 waveform involved in passive feeding. However, the durations of single El, E1 followed by sustained E2 and numbers of E2 were decreased by high-dose Zn^2＋ stress. Meanwhile, the feeding behavior of S. avenae was influenced by Zn^2＋ and could produce the accumulated effects under the stress of high-dose Zn^2＋. However, the feeding behavior of S. avenae could be promoted by low dose of Zn^2＋. Based on that, the effective concentration was 400 mg/kg. The Zn^2＋ stress have changed the feeding behavior of S. avenae since the 5^th and 10^th generations. That was resulted from the total durations and C were extended and increased, while the number of E2 was decreased. These findings will provide for controlling the damage from S. avenae under Zn^2＋ contamination conditions and numbers of np a theoretical basis
出处
《生态学报》
CAS
CSCD
北大核心
2016年第9期2537-2543,共7页
Acta Ecologica Sinica
基金
国家自然科学基金项目(39970112,30470268)
高校博士点基金(2013020411000)
