摘要
目的:建立复合应激方法诱导的肝郁脾虚证小鼠模型并对其进行评价。方法:80只CS7BL/6J小鼠随机分为正常组、模型组、逍遥散组和氟西汀组,每组20只。除正常组外其余各组小鼠均以复合应激方法进行造模,观察小鼠宏观表征、体质量、摄食量、行为学和血清D-木糖含量,并对小鼠的肝郁脾虚的程度进行评价。结果:1宏观表征:与正常组比较,模型组小鼠其精神状态出现易怒最后转变为低落,斗争减少,毛发凌乱无光泽,扎堆少动,灌胃抵抗减弱,出现便溏等,且体质量和摄食量均显著减少(P<0.05),逍遥散对模型小鼠的宏观表征有一定的改善作用。2旷场实验:与正常组比较,模型组小鼠中央区进入次数和总运动距离显著减少(P<0.01),与模型组比较,逍遥散组和氟西汀组中央区停留时间明显降低(P<0.05,P<0.01),总运动距离明显增加(P<0.01,P<0.05)。3新环境抑制进食实验:各组小鼠新环境抑制进食时间无显著差异。4高架十字迷宫实验:与正常组比较,模型组小鼠开放臂停留时间比例显著减少(P<0.01),与模型组比较,逍遥散组小鼠开放臂停留时间比例显著增加(P<0.05);5血清D-木糖含量:与正常组比较,模型组小鼠血清D-木糖含量显著减少(P<0.05),与模型组比较,逍遥散组小鼠血清D-木糖含量显著增加(P<0.01),且高于氟西汀组(P<0.05)。结论:本研究采用复合应激方法制作小鼠肝郁脾虚证模型的初步尝试是成功的。
Objective： To evaluate the mouse models with syndrome of stagnation of liver qi and spleen deficiency induced by multiple stresses. Methods： Sixty C57BL/6J mice were randomly divided into control group, model group, Xiaoyaosan group and fluoxetine group, and with 15 mice in each group. All the mice except control group received multiple stresses, in order to establish the mice models with syndrome of stagnation of liver qi and spleen deficiency. The macroscopic characters, body weight, food consumption, behavioristics and contents of serum D-Xylose were observed and the degrees of stagnation of liver qi and spleen deficiency of mice were evaluated. Results： 1Compared with control group, the macroscopic characters of mice in model group manifested as follows： the irritable mental state was appeared in mice of model group, and turned into depression finally; the tussle among mice was induced; the hair of mice was disheveled and lackluster; the gavage resistance was weakened; the sedentary behavior and loose stool were appeared; all the mice in model group liked gathering together, the body weight and food consumption were reduced significantly（P〈0.05）. Xiaoyaosan could improve the macroscopic characters of mice models. 2Compared with control group, the observation indexes of open field test of mice in model group were changed significantly such as the central zone entries and duration time were reduced（P〈0.05）, while the difference between control group and Xiaoyaosan group was not significant. 3The difference in suppressed feeding time of novelty suppressed feeding test among the 4 groups was not significant. 4Compared with control group, the open-arm duration time of elevated plus-maze test of mice in model group was reduced significantly（P〈0.01）, while the difference between control group and Xiaoyaosan group was not significant. 5Compared with control group, the contents of serum D-Xylose of mice in model group was reduced significantly（P〈0.05）, while the difference between control group and Xiaoyaosan group was not significant. Conclusion： The preliminary try on establishing mouse models with syndrome of stagnation of liver qi and spleen deficiency by using multiple stresses in this study was successful.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2016年第5期1840-1844,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81473597)
北京市自然科学基金项目(No.7152093)~~
关键词
肝郁脾虚证
复合应激
动物模型
评价
逍遥散
Syndrome of stagnation of liver qi and spleen deficiency
Multiple stresses
Animal model
Experimental research
Xiaoyao Powder
