摘要
目的研究ω-3脂肪酸对呼吸机相关性肺损伤（VILI）大鼠炎性反应的影响,探讨ω-3脂肪酸对于VILI的预防作用及其机制。方法 48只SD大鼠按随机数字表法分成对照组（C组）,VILI模型组（M组）,ω-3脂肪酸干预1、2、3、4组（I1组、I2组、I3组、I4组,制模前0.5h分别静脉注射ω-3脂肪酸60、120、180、240mg/kg）。除C组外各组均行机械通气,3h后处死大鼠。取肺组织测定肺湿/干重（W/D）比值、肺组织髓过氧化物酶（MPO）水平;收集支气管肺泡灌洗液（BALF）测定总蛋白水平;取血测定白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）水平。结果与M组比较,随着ω-3脂肪酸用量增加,I1、I2、I3、I4组肺W/D比值下降（P〈0.01）;BALF中总蛋白水平、MPO水平、血TNF-α水平逐渐降低（P〈0.01）;血IL-10水平升高（P〈0.05）。结论ω-3脂肪酸可通过抗炎、抗氧化应激、减少渗出等机制发挥对肺的保护作用,还可减轻VILI,其最适剂量范围为120-180mg/kg。
Objective To invest the influence of omega-3 fatty acids in inflammatory reaction in rats with ventilator induced lung injury（VILI）and to explore the mechanism of preventive effect of omega-3 fatty acids in VILI.Methods Forty-eight Sprague-Dawley（SD）rats were randomly divided into control group（group C）,VILI model group（group M）,omega-3 fatty acids intervention group 1,2,3,4（group I1,I2,I3,I4,with intravenous injection of omega-3 fatty acids 60,120,180,240mg/kg respectively,half an hour before models reproduced at 3hours postdose）.All rats except group C were suffered mechanical ventilation,all rats were sacrificed.The lung tissue was collected and the wet-to-dry weight ratio（W/D）and myeloperoxidase（MPO）were determined.Bronchoalveolar lavage fluid（BALF）was collected and total protein content was evaluated.Blood was collected and interleukin-10（IL-10）,tumor necrosis factor（TNF-α）were measured.Results Compared with group M,W/D of lung in group I1,I2,I3,I4 decreased in dose-dependence（P〈0.01）.The total protein in BALF,activity of MPO and content of TNF-αwas gradually reduced in dose-dependence（P〈0.01）.The content of IL-10 was upgraded（P〈0.05）.Conclusion Omega-3 fatty acids could protect lung tissue and alleviate VILI through anti-inflammatory,anti-oxidative stress and the decreased of effusion.The best dose of omega-3 fatty acids is 120-180mg/kg.
出处
《重庆医学》
CAS
北大核心
2016年第14期1896-1899,共4页
Chongqing medicine
关键词
脂肪酸类
ω3
肺炎
呼吸机相关性
炎性反应
氧化应激
fatty acids
omega-3
pneumonia
ventilator-associated
inflammatory reaction
oxidative stress
