摘要
目的探讨骨髓细胞形态学与流式细胞在检测B细胞非霍奇金淋巴瘤(B-NHL)骨髓受累中的价值。方法 B-NHL患者同时做骨髓涂片和流式细胞检测。骨髓涂片经瑞士吉姆萨染色,分类计数骨髓细胞;流式细胞检测先处理样本后加荧光染色,上机处理。结果 93例B-NHL患者经骨髓涂片检测阳性者为11例,阳性率为11.8%;经流式细胞检测阳性者为27例,阳性率为29.0%,两种方法比较差异有统计学意义(P<0.05)。在11例骨髓涂片阳性的样本中,有1例经流式细胞检测为阴性。结论骨髓涂片和流式细胞检测都是诊断B-NHL骨髓受累的重要方法,两者各有优势,联合运用两种方法可为诊断B-NHL骨髓受累提供更准确的依据。
Objective To explore the value of combined detection of bone marrow smear（BMS）and flow cytometry（FCM）to examine bone marrow involvement（BMI）of B cell non-Hodgkin′s lymphoma（B-NHL）.Methods Patients with B-NHL were detected by BMS and FCM simultaneously.Smears were stained with Wright-Giemsa′s staining to differentially count neoplastic cells and the processed sample with fluorescent staining for the FCM.Results 93 cases of patients with B-NHL were detected.The detection rate of BMS and FCM were 11.8% and 29.0%.There was statistical difference of detection rate between the two method（P〈0.05）.In 11 cases patients of BMI with the method of BMS,while using the method of FCM 10 cases were positive.ConclusionBoth BMS and FCM are useful to diagnose BMI of B-NHL,each of them has individual characteristics.Combined detection of these two methods can increase the diagnostic of BMI.
出处
《国际检验医学杂志》
CAS
2016年第9期1175-1176,1179,共3页
International Journal of Laboratory Medicine
基金
新疆医科大学科研创新基金资助项目(XJC201390)
关键词
非霍奇金淋巴瘤
流式细胞检测
骨髓受累
non-Hodgkin′s lymphoma
flow cytometry
bone marrow involvement
