摘要
为探讨不同基因型的玉米单倍体雄穗自然加倍的情况,利用由7个优良自交系组配的FAPW/BN490A、BN490A/BN486A、PHZ51/H33和MT-1/H32等4个单倍体群体研究玉米单倍体的花药外露与花粉育性。结果表明：FAPW/BN490A群体位于雄穗分枝的花药外露概率高于位于雄穗主轴的花药外露概率;在所研究的4个单倍体群体中,FAPW/BN490A群体的花药外露率和可育花粉率均为最高,平均值分别为35.7%和72.2%;其次为BN490A/BN486A群体（13.0%,53.8%）与PHZ51/H33群体（13.0%,48.7%）,MT-1/H32群体的最低,分别为12.0%和47.4%;通过多重比较发现FAPW/BN490A群体的花药外露率和花粉可育率与其余3个群体均显著差异,但其余3个群体间无差异,表明玉米单倍体的雄穗育性只在特定基因型间存在差异;在4个单倍体群体花药不外露株的植株中,均存在一定比例可育的花药,花粉可育率为0~95.0%,且每个群体可育花粉率在〉0~25.0%和〉25.0%~50.0%的植株较多,而〉50.0%~75.0%和〉75.0%的植株较少,即不同基因型的玉米单倍体群体中,花药不外露株的雄穗育性有较低程度恢复,且可育花粉率越高的植株数越少。
To explore whether the male fertility of maize haploid is affected by genotypes,anther extrusion and pollen fertility in four haploid populations derived from seven elite inbred lines were investigated.The probability of extruded anther located in central spike was higher than that in lateral branches in FAPW/BN490 Ahaploid population.Both the rate of haploid plants with extruded anther and fertile pollens were the highest in FAPW/BN490 Ahaploid population,which reached 35.7% and 72.2%,respectively,followed by BN490A/BN486 Ahaploid population（13.0%,53.8%）,PHZ51/H33 haploid population（13.0%,13.0%）,and MT-1/H32 haploid population（12.0%,47.4%）.Multiple comparisons indicated that both the rate of haploid plants with extruded anther and fertile pollens had significant differences between FAPW/BN490 Aand the remaining three haploid populations,but none among the remaining three haploid populations.It suggested that the male fertility of maize haploid varied among some genotypes,but not all.There was a certain percentage of anther with fertile pollen in some plants with unextruded anther in four haploid populations,and the fertile pollen rate varied from 0to 95.0%,and the number of plants with fertile pollen rate ranging from 0to 25.0% and 25.0% to 50.0%is larger than those ranging from 50.0% to 75.0% and more than 75.0%.Plants with unextruded anther have a lower level of spontaneous chromosomal doubling potential in different genotypes of maize haploid,and higher fertile pollen rate of the less number of plants.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2016年第6期1-8,共8页
Journal of China Agricultural University
基金
国家国际科技合作专项项目(2014DFG31690)
关键词
玉米
单倍体
花药外露
花粉育性
自然加倍
maize
haploid
anther extrusion
pollen fertility
spontaneous chromosomal doubling
