摘要
能量状态与采后水果的成熟、衰老及生理失调紧密相关。为了研究NO处理在桃果实冷藏期间对其能量代谢的调控机理,选取八成熟'肥城桃'果实,分别用去离子水(对照)、5、15、30μmol·L-1NO溶液和5μmol·L-1c-PTIO(NO清除剂)溶液处理后于0℃下恒温贮藏。分别在果实贮藏当天、第1、2、3、4、5周测定不同处理'肥城桃'果实的冷害指数、硬度、丙二醛(MDA)、过氧化氢、三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、单磷酸腺苷(AMP)的含量和能荷水平的变化,以及琥珀酸脱氢酶(SDH)、细胞色素氧化酶(CCO)、氢离子ATP酶(H+-ATPase)、钙离子ATP酶(Ca2+-ATPase)等线粒体呼吸代谢关键酶的活性变化情况。结果表明:在冷藏过程中,桃果实内ATP、ADP含量,能荷水平及桃果实线粒体内SDH、CCO、H+-ATPase、Ca2+-ATPase活性逐渐降低,而AMP、过氧化氢及MDA含量升高;与对照相比,NO处理能够显著延缓桃果实内ATP、ADP、能荷含量及能量代谢酶活性的降低,抑制过氧化氢、MDA、AMP含量的上升,其中以15μmol·L-1NO处理桃果实最为显著,而NO清除剂c-PTIO处理则加剧了桃果实ATP、能荷含量及能量代谢酶活性的下降以及过氧化氢、MDA、AMP含量的上升。上述结果表明,15μmol·L-1外源NO处理能有效维持桃果实在冷藏期间的能量代谢,延缓膜脂过氧化过程,提高桃果实的抗冷性,而c-PTIO处理则促进了冷藏期间桃果实能量代谢紊乱,加剧了桃果实的冷害程度。
The processes of ripening,senescence and physiological disorder of postharvest fruit are associated with energy status.To explore the energy regulation mechanism of nitric oxide on peach fruits during cold storage,‘Feicheng’peach fruits at physiological mature were treated with deionized water(control),5,15,30μmol·L-1 NO and 5μmol·L-1 c-PTIO solution(NO scavenging agent)respectively,and then stored at 0℃.The chilling injury index,firmness,and contents of malondialdehyde(MDA),hydrogen peroxide,adenosine triphosphate(ATP),adenosine diphosphate(ADP),adenosine monophosphate(AMP),energy charge and changes of activities of key enzymes in respiratory metabolism of mitochondria,such as H+-ATPase,Ca2+-ATPase,succinate dehydrogenase(SDH),cytochrome oxidase(CCO),in‘Feicheng’peach fruits were measured regularly at 0,1,2,3,4,5 weeks during the storage.The results showed that,the contents of ATP,ADP,energy charge and the activities of SDH,CCO,H+-ATPase,Ca2+-ATPase reduced,while the contents of AMP,H2 O2 and MDA increased in peach fruits during the storage.Compared to the control group,NO treatment significantly delayed the decrease of contents of ATP,energy charge and the activities of SDH,CCO,H+-ATPase,Ca2+-ATPase,and inhibited the increase of contents of AMP,H2 O2 and MDA in peach fruits during treatment of 15μmol·L-1 NO showed better effect than other treatment.However,c-PTIO treatment intensified the decrease of contents of ATP,energy charge and the activities of SDH,CCO,H+-ATPase,Ca2+-ATPase and the increase of contents of H2 O2,MDA and AMP in peach fruits during the cold storage.These results suggested that 15μmol·L-1 NO could effectively maintain the energy metabolism,delay membrane lipid peroxidation and improve the cold resistance of peach fruits during the cold storage,while c-PTIO treatment could promote the disorder of energy metabolism,and aggravate the degree of chilling injury of peach fruits during the cold storage.
作者
张小康
上官相超
陈长宝
张丽丽
周杰
朱树华
ZHANG Xiao-kang;SHANGGUAN Xiang-chao;CHEN Chang-bao;ZHANG Li-li;ZHOU Jie;ZHU Shu-hua(College of Chemistry and Materials Science,Shandong Agricultural University,Tai’an 271018,China)
出处
《保鲜与加工》
CAS
北大核心
2019年第4期1-9,共9页
Storage and Process
基金
国家自然科学基金项目(31770724)
关键词
桃
能量代谢
一氧化氮
酶活性
三磷酸腺苷
能荷
Prunus persica
energy metabolism
nitric oxide
enzyme activity
adenosine triphosphate(ATP)
energy charge
