摘要
目的探讨1%卢立康唑乳膏单纯制剂的体外抗真菌谱与抗真菌活性,并与2%酮康唑乳膏、1%盐酸特比萘芬乳膏及1%萘替芬-0.25%酮康唑乳膏进行对比。方法选用临床分离致病真菌及马拉色菌标准株共14种32株,用琼脂扩散法行药敏试验。配制含2%琼脂培养基的含菌培养皿,打孔后分别加入上述4种乳膏,培养7d后测定各含药孔周围的抑菌圈直径并进行比较。结果 1%卢立康唑乳膏对各实验菌株均形成抑菌圈,抑菌圈直径均数为50.35mm。1%特比萘芬乳膏对除念珠菌和镰刀菌以外的实验菌株的抑菌圈直径均数为49.24 mm。2%酮康唑乳膏对除镰刀菌以外的实验菌株的抑菌圈直径均数为33.22mm。1%萘替芬-0.25%酮康唑乳膏对各实验菌株的抑菌圈直径均数为52.46mm。1%卢立康唑乳膏的抑菌圈直径均数与2%酮康唑乳膏及1%特比萘芬乳膏相比,差异均有统计学意义(P=0.000),而与1%萘替芬-0.25%酮康唑乳膏无明显差异(P>0.05)。结论 1%卢立康唑乳膏与1%萘替芬-0.25%酮康唑乳膏体外抗真菌谱及抗真菌活性相近,且强于2%酮康唑乳膏及1%特比萘芬乳膏。
Objective To research the anti-fungal spectrum and activity of the 1%luliconazole cream compared with other three creams that contain of 2% ketoconazole,of 1% terbinafine,of 1% naftifine-0.25% ketoconazole cream respectively.Methods Thirty-two isolates of pathogenic fungi belonging to 14 species isolated from clinic and three species of Malassezia standard stains were enrolled into the experiment.The agar diffusion method was used to judge drug sensitivity.Organism suspension of each species was spread on the surface of the plate of the optimal media containing 2% agar.Then wells were made in the plate and four types of cream were put in each well respectively.After seven-day incubation,the diameter of the inhibition zone around the well full of each cream was observed and recorded.Results The inhibition zone around the well filled with 1%luliconazole cream for all experiment isolates was observed,with the mean diameter of 50.35 mm.Similarly,the mean diameter of inhibition zone of 1% naftifine-0.25% ketoconazole cream for all experiment isolates was 52.46 mm.About1%terbinafine cream,the mean diameter was 49.24 mm but there was no inhibition zone observed around Candidaspp.and Fusarium solani.As for 2% ketoconazole cream,the mean diameter was 33.22 mm but there was no inhibition zone observed around Fusarium solani.There were no significant significances for mean diameters of the inhibition zone when comparing1%luliconazole cream with 1% naftifine-0.25% ketoconazole cream,but when comparing 1% luliconazole cream with 2%ketoconazole cream and 1%terbinafine cream,the difference was statistically significant（P=0.000）.Conclusion The antifungal spectrum and activity of 1%luliconazole cream are nearly the same as that of 1% naftifine-0.25% ketoconazole cream,and is stronger than that of 2% ketoconazole cream and 1%terbinafine cream.
出处
《中国真菌学杂志》
CSCD
2016年第2期90-94,共5页
Chinese Journal of Mycology
关键词
1%卢立康唑乳膏
抗真菌谱
抗真菌活性
体外
1%luliconazole cream
anti-fungal spectrum
anti-fungal activity
in vitro
