摘要
为了解中国河南省小麦田常见杂草野燕麦上的病原真菌种类,以期为更好的利用植物病原真菌资源和开发生物除草剂奠定基础。采用组织分离法对野燕麦病害样品进行分离,结合形态观察和r DNA ITS序列分析对菌株进行鉴定,最后用人工接种法对部分菌株进行致病性测定。从81份自然发病样品中分离到157个菌株,共鉴定了9个属的17种真菌。对于做致病性测定的28个菌株,其中新月弯孢菌株YM1362对供试杂草表现出很强致病性,而对棉花、大豆、花生等植物没有致病性。结果表明野燕麦上的病原真菌主要属于链格孢属、弯孢属、平脐蠕孢属和凸脐蠕孢属;其中菌株YM1362具有开发为防治双子叶作物田中禾本科杂草的生物除草剂的潜力。
In order to understand the types of pathogenic fungi on wild oats（Avena fatua L.） in Henan Provinceand provide basic information for the utilization of pathogenic fungal resources on weeds and the developmentof microbial herbicides,samples were isolated by tissue isolation method.The isolates were identified bymorphological observation and alignments of r DNA ITS sequence,and the pathogenicity of some isolates wastested by artificial inoculation.A total of 17 species of 9 genera were identified among 157 strains isolated from81 samples.Among the 28 strains for pathogenicity detection,the Curvularia isolate YM1362 showed strongpathogenicity for the tested weeds,but no pathogenicity for cotton,soybean,peanut and other plants.Theresults indicated that the main pathogenic fungi on wild oats belonged to Alternaria,Curvularia,Bipolaris and Exserohilum;and the isolate YM1362 had the potential for developing biological herbicides for preventingPoaceae weed in dicotyledon field.
出处
《中国农学通报》
2016年第15期166-170,共5页
Chinese Agricultural Science Bulletin
基金
河南省教育厅自然科学研究计划重点项目"小麦返白系白化相关基因aidpt的克隆及功能研究"(14A180006)
河南科技大学博士科研启动基金(4024-13480053)
(4026-13480034)
河南科技大学青年基金"牡丹内生真菌的多样性研究"(4024-13350067)
关键词
野燕麦
病原真菌
致病性
Avena fatua
pathogenic fungi
pathogenicity
