摘要
目的:建立双波长HPLC测定诃子中莽草酸、没食子酸和鞣花酸含量的方法。方法:采用Agilent C18柱(250mm×4.6mm,5μm),以甲醇-0.1%磷酸为流动相梯度洗脱,流速0.8m L/min,检测波长214nm(莽草酸)和270nm(没食子酸和鞣花酸),柱温25℃。结果:莽草酸、没食子酸、鞣花酸分别在各自进样量范围内与峰面积呈良好线性关系,相关系数均在0.9992以上;各成分的平均回收率均在97.1%-97.5%(n=6),RSD均小于2.0%。结论:该方法快速、准确、简便,可用于诃子中莽草酸、没食子酸及鞣花酸的含量测定。
Objective:To establish method for simultaneous determination of shikimic acid, gallic acid and ellagic acid of Fructus Chebulae by dual-wavelength HPLC.Methods:An Agilent C18 column(250mm×4.6mm, 5μm) was used with a mobile phase of methanol-0.1% phosphoric acid at 0.8m L/min by gradient elution, and the detection wavelengths were set at 214nm(shikimic acid) and 270nm(gallic acid and ellagic acid) with the column temperature of 25℃.Results:Shikimic acid, gallic acid and ellagic acid had good linear relation with peak area respectively in the concentration ranges oftheir individual sample size(r>0.999 2), and the overall average recovery rates were in the range of 95.0%-100.5%(n=6) with RSD less than 2.0%.Conclusion:The results indicated that this dual-wavelength HPLC method is rapid, accurate and simple, which can be used for the determination of shikimic acid, gallic acid and ellagic acid in Fructus Chebulae.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2017年第2期819-821,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
辽宁省博士启动基金(No.201501097)~~
