摘要
目的:采用核磁共振氢谱技术(~1H NMR)研究电针胃经穴对应激性胃溃疡大鼠胃黏膜组织代谢物谱的表达。方法:40只SD大鼠随机分为正常组、模型组、胃经组和胆经组。利用束缚-冷应激法制备应激性胃溃疡模型大鼠,光镜下观察大鼠胃黏膜组织病理形态学的变化,采用~1H NMR技术获取胃黏膜组织~1H NMR谱,并利用模式识别方法分析各组间的代谢物谱的差异。结果:与正常组比较,模型组大鼠胃黏膜损伤和胃黏膜组织代谢轮廓差异明显,胃黏膜组织乙酸、肌醇、磷酸胆碱、谷氨酸、甘氨酸、乙醇胺、赖氨酸、3-羟基丁酸、缬氨酸、异亮氨酸、牛磺酸、亮氨酸和甘露醇含量上升(P<0.05),乳酸、甲硫氨酸和天冬氨酸含量下降(P<0.05);与模型组比较,胃经组和胆经组大鼠胃黏膜得到明显修复,胃经组大鼠胃黏膜组织代谢轮廓更接近正常大鼠,胃黏膜组织甲硫氨酸、天冬氨酸含量上升(P<0.05),乙酸、肌醇、磷酸胆碱、谷氨酸、甘氨酸、赖氨酸、异亮氨酸、牛磺酸、乳酸、亮氨酸和甘露醇含量下降(P<0.05);胆经组大鼠胃黏膜组织3-羟基丁酸、甲硫氨酸含量上升(P<0.05),肌醇、磷酸胆碱、谷氨酸、乙醇胺、异亮氨酸、乳酸、亮氨酸和甘露醇含量下降(P<0.05)。结论:电针胃经穴可调节胃溃疡大鼠胃黏膜组织特异代谢物的表达,促进胃黏膜损伤的修复。
Objective: To research the effects of electropuncture of acupoints on the foot Yangming stomach meridian on metabolic profiling of gastric mucosa in rats of gastric ulcer induced by stressusing the ~1H nuclear magnetic resonance(~1H NMR). Methods: Forty SD rats were randomly divided into the control group, the model group, the stomach meridian group and the gallbladder meridian group. The rat model of gastric ulcer was induced by the restrained plus cold stress. The pathological changes in the rat gastric mucosa tissue were observed by light microscope. The ~1H NMR spectroscopy was determined with the ~1H NMR technique. And, meanwhile the differences in the metabolic profiling were identified with the pattern recognition method. Results: Compared to normal group, the gastric mucosa damage and gastric mucosa metabolic profiling are obvious, the concentration of acetic acid, choline, inositol phosphate, glutamic acid, glycine, ethanolamine, lysine, 3-hydroxy butyric acid, valine, isoleucine, taurine, leucine and mannitol increase and the concentration of lactic acid, methionine and aspartic acid decrease in model group rats(P<0.05). Compared to model group, the gastric mucosa damage have been recovered in stomach meridian group and gallbladder meridian group, the metabolic profiling of gastric mocusa tissue in stomach meridian group are similar to the normalgroup. The concentration of methionine, aspartic acid increase and acetic acid, choline, inositol phosphate, glutamic acid, glycine, lysine, lactic acid, leucine, isoleucine, taurine, and mannitol decrease in stomach meridan group(P<0.05). The concentration of 3-hydroxy butyric acid, methionine increase and choline, inositol phosphate, lactic acid, leucine, isoleucine, glutamic acid, ethanol amine and mannitol decrease in gallbladder meridian group(P<0.05). Conclusion: The electropuncture of acupoints on the foot Yangming stomach meridian could regulate the metabolic profiling of gastric mucosa, resulting in improving the gastric mucosa repair of gastric ulcer rats.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2017年第3期1033-1036,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81473751
No.81260556)
国家重点基础研究发展计划(973计划)项目(No.2015CB554502)
广东省自然科学基金项目(No.2014A030313005)
深圳市科技计划项目(No.JCYJ20160406140612883)~~
关键词
电针
胃经穴
胃溃疡
核磁共振
代谢组
Electropuncture
Acupoints on the foot Yangming stomach meridian
Gastric ulcer
Nulcear magnetic resonance(NMR)
Metabolome
