摘要
目的:采用核磁共振氢谱技术(1H NMR)研究电针胃经穴治疗应激性胃溃疡大鼠大脑皮质、下丘脑、延髓代谢物谱的表达。方法:40只Wistar大鼠随机分为正常组、模型组、胃经组和胆经组。利用束缚冷应激法制备应激性胃溃疡模型大鼠,造模成功后用电针分别刺激胃经组和胆经组相关穴位。收集大鼠大脑皮质、下丘脑、延髓,获取一维1H NMR谱,并利用模式识别方法分析各组间的代谢轮廓差异。结果:模型组与正常组、胃经组、胆经组大鼠大脑皮质、下丘脑和延髓代谢物谱均有明显区分,其中正常组与模型组分离最明显,胃经组最接近正常组,说明其代谢物谱趋向正常。与模型组比较,胃经组大鼠大脑皮质乳酸、萘乙酸、甘露醇等表达上升(P<0.05),而肌酸、腺苷、肌苷等表达下降(P<0.05),下丘脑乳酸、肌醇、谷氨酸酯等表达上升(P<0.05),γ-氨基丁酸等表达下降(P<0.05),延髓乳酸、谷氨酸脂、肌醇、丙氨酸等表达上升(P<0.05),而萘乙酸、肌酸等表达下降(P<0.05)。结论:电针胃经穴可调节胃溃疡大鼠大脑皮质、下丘脑、延髓的代谢产物表达,大脑皮质、下丘脑和延髓是电针胃经穴治疗胃溃疡的中枢响应位点。
Objective: To study the metabolic profiling of the cerebral cortex, hypothalamus and medulla oblongata from irritable gastric ulcer rats treated with electroacupuncture on acupoints of the stomach meridian with 1H nuclear magnetic resonance(1H NMR) spectroscopy. Methods: Forty SD rats are randomly divided into the control group, the model group, the stomach meridian group and the gallbladder meridian group. The irritable gastric ulcer rat model was established by water-immersion and restraint stress method. After modeling, the rats from the stomach meridian group and the gallbladder meridian group were treated with electroacupuncture on the stomach meridian and gallbladder meridian respectively. 1H NMR spectroscopy was applied to detect the metabolic profiling of the cerebral cortex, hypothalamus and medulla oblongata, then analyzed metabolic contour differences among the groups using pattern recognition method. Results: Obvious distinctions were found between the metabolites spectrum of the cerebral cortex, hypothalamus and medulla oblongata from the model group, the normal group, the stomach meridian group and the gallbladder meridian group. More significant differences were found between the normal group and the model group, while the metabolites spectrum of the stomach meridian group was more distinctly close to that in the normal group, indicating that metabolites spectrum of the stomach meridian group was likely to normal. Compared with the model group, the concentration of lactic acid, acetic acid, mannitol increased while creatine, adenosine and inosine decreased in cerebral cortex of the stomach meridian group(P<0.05), the concentration of lactic acid, glutamic acid, inositol and alanine increased while gamma aminobutyric acid decreased in hypothalamus of the stomach meridian group(P<0.05), the concentration of lactate, glutamate, myo-lnositol, alanine increased(P<0.05) and naphthylacetic acid and creatine decreased in medulla oblongata of the stomach meridian group(P<0.05). Conclusion: Electroacupuncture on acupoints of the stomach meridian could regulate specific metabolites in the cerebral cortex, hypothalamus and medulla oblongata, indicating that the cerebral cortex, hypothalamus and medulla oblongata as the central response site in the treatment of gastric ulcer by electroacupuncture on acupoints of the stomach meridian.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2017年第7期3191-3195,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81473751
No.81260556)
广东省自然科学基金项目(No.2014A030313005)
深圳市科技计划项目(No.JCYJ20160406140612883)~~
关键词
电针
胃溃疡
核磁共振
代谢组
中枢机制
胃经
Electroacupuncture
Gastric ulcer
Nuclear magnetic resonance
Metabolome
Central mechanism
Stomach meridian
