摘要
目的:观察艾灸干预胃溃疡大鼠胃黏膜相关脑肠肽的动态表达,探讨艾灸干预胃溃疡的时效关系。方法:SD大鼠72只随机分为3批,各批分为正常组、模型组、胃经穴位组和非经穴位组(n=6)。采用70%乙醇灌胃复制胃溃疡大鼠模型。胃(非)经穴位组的大鼠进行艾灸1、4、7d(30min/d)。干预结束后于光镜下观察胃黏膜形态,RT-PCR测定胃黏膜增殖细胞核抗原(PCNA)及P物质(SP)表达。结果:与正常组比较,模型组大鼠胃黏膜溃疡灶明显,胃黏膜PCNA表达降低(P<0.05),SP表达升高(P<0.05);与模型组比较,干预1d后胃经穴位组大鼠胃黏膜PCNA表达升高(P<0.05),干预4d后胃经穴位组PCNA表达升高(P<0.05),SP降低(P<0.05);干预7d后各组大鼠胃黏膜PCNA及SP表达无显著差异。结论:艾灸胃经穴位能有效调节相关脑肠肽指标,促进胃黏膜损伤修复,并具有一定的时效关系。
Objective: To observe the effects of moxa-wool moxibustion on dynamic expression of brain-gut peptide in gastric mucosa of rats with gastric ulcer, and to explore the time effect relationship of moxa-wool moxibustion in treating gastric ulcer. Methods: Seventy-two SD rats were randomly divided into three batches. Each batches was divided into control group, model group, stomach meridian acupoint group and non acupoints group(n=6). Rats model of gastric ulcer were established by 70% ethanol intragastric administration. The stomach meridian acupoint group and non acupoint moxibustion group were treated with moxa-wool moxibustions in 1 d, 4 d, 7 d(30 min/d). After intervention, morphology of gastric mucosa was observed under light microscope. The expressions of proliferating cell nuclear antigen(PCNA) and substance P(SP) in gastric mucosa were measured by RT-PCR. Results:Compared with the control group, the gastric mucosal ulcer in the rats of model group was obvious, the expression of PCNA in the gastric mucosa singnificantly decreased(P<0.05), and the expression of SP singnificantly increased(P<0.05). Compared with the model group, the PCNA expression in gastric mucosa of stomach meridian acupoints group increased after moxa-wool moxibustion in 1 d(P<0.05). After moxa-wool moxibustion 4 days, the expression of PCNA in stomach meridian acupoints group was increased(P<0.05), and SP was decreased(P<0.05). After moxa-wool moxibustion 7 days, there was no significant difference in the expression of PCNA and SP in each group. Conclusion: Moxa-wool moxibustion on the stomach meridian acupoints could effectively regulate the related brain gut peptide index, promote the repair of gastric mucosal injury, and has certain time-effect relationship.
作者
何其达
刘密
连林宇
张媛
沈佳成
刘彩春
黄妙森
张珑滨
钱林超
常小荣
杨宗保
HE Qi-da;LIU Mi;LIAN Lin-yu;ZHANG Yuan;SHEN Jia-cheng;LIU Cai-chun;HUANG Miao-sen;ZHANG Long-bin;QIAN Lin-chao;CHANG Xiao-rong;YANG Zong-bao(Shenzhen Research Institute,Xiamen University,Shenzhen 518000,China;Medical College,Xiamen University,Xiamen 361102,China;College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou 350000,China;College of Acupuncture-moxibustion and Massage,Hunan University of Chinese Medicine,Changsha 410000,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2019年第3期943-946,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
深圳创新科技计划项目(No.JCYJ20160406140612883)
国家自然科学基金项目(No.81473751)
国家重点基础研究发展计划(973计划)(No.2015CB554502)~~
关键词
艾灸
胃溃疡
胃经
时效关系
脑肠肽
Moxa-wool moxibustion
Gastric ulcer
Stomach meridian
Time-effect relationship
Brain-gut peptide
