摘要
目的:研究热量控制对肥胖大鼠白色脂肪组织(WAT)中瘦素以及糖脂代谢的影响。方法:Wistar雄性大鼠10只为正常组(N组)给予普通饲料喂养,另20只高脂饲料喂养造模,造模成功后通过随机数字表法分为模型组(M组)和热量控制组(CR组),各10只。除CR组每只给予M组同龄大鼠摄食量的70%,其他两组大鼠均自由进食饮水,共干预8周。检测各组大鼠体质量和血清葡萄糖(FPG)、胰岛素(INS)、总胆固醇(TC)、甘油三酯(TG)及瘦素的含量,并检测WAT中瘦素的蛋白表达。结果:干预8周后,与M组相比,CR组体质量、血清FPG、INS、TG、TC及瘦素含量均显著下降(P<0.01),且WAT中瘦素蛋白的表达水平显著降低(P<0.01)。结论:热量控制能减轻肥胖大鼠体质量,改善糖脂代谢,降低血清瘦素水平,下调WAT中瘦素的蛋白表达。
Objective:To observe the effect of caloric restriction on the expression of leptin in white adipose tissue(WAT)and glycolipid metabolism of obese rats.Methods:A total of 10 male Wistar rats as normal control group(N group)fed with normal diet,the other 20 rats with obesity induced with a high-fat diet were randomly divided into model group(M group)and caloric restriction group(CR group),each group were 10 rats.The CR group was fed only 70%of high-fat diet in the M group,the rats of N group and M group were fed with free eating and drinking,the intervention lasted for 8 weeks.The rats’body weight and the level of serum FPG,INS,TG,TC,leptin,and the expression of leptin in WAT were detected.Results:Compared with M group,body weight and the level of serum FPG,INS,TG,TC and leptin were significantly decreased in CR group(P<0.01).And the expression of leptin in the WAT decreased significantly in CR group(P<0.01).Conclusion:Caloric restriction can effectively reduce the body weight and regulate glycolipid metabolism of obese rats.Besides,it also can reduce the level of serum leptin and the expression of leptin in WAT.
作者
王雅媛
梁凤霞
WANG Ya-yuan;LIANG Feng-xia(College of Acupuncture and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430061,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2019年第7期3247-3250,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金面上项目(No.81473787)~~
关键词
热量控制
肥胖
白色脂肪
瘦素
糖脂代谢
Caloric restriction
Obesity
White adipose tissue
Leptin
Glucolipid metabolism
