摘要
目的分析高胰岛素对MCF-7/ADR细胞P-糖蛋白(P-gp)表达和功能的影响,并初步探讨胰岛素对MCF-7/ADR细胞化疗敏感性的影响。方法用不同胰岛素浓度(0.001、0.005、0.01、0.05、0.10μmol/L)的全细胞培养基干预MCF-7/ADR细胞,分别采用Real-time PCR法检测细胞中P-gp m RNA表达,Western Blot法检测细胞中P-gp蛋白表达水平,罗丹明123荧光实验测定P-gp的外排功能变化,MTT法检测MCF-7/ADR细胞活性及化疗敏感性。结果 0.10μmol/L的胰岛素可促进MCF-7/ADR细胞增殖,0.05及0.10μmol/L浓度的胰岛素可增加MCF-7/ADR细胞P-gp m RNA及蛋白水平表达,并能增加P-gp的外排功能,降低MCF-7/ADR细胞对表阿霉素的化疗敏感性。结论高浓度胰岛素可能通过促进MCF-7/ADR细胞P-gp的表达和功能,增加乳腺癌细胞化疗耐药性,从而降低MCF-7/ADR细胞的化疗敏感性。
Objective To analyse the effects of high insulin on the expression and function of P-glycoprotein （P-gp）, and preliminarily investigate the influence of insulin on chemotherapeutic sensitivity in MCF- 7/ADR cells. Methods MCF-7/ADR cells were cultured with different concentrations of insulin （0.001, 0.005, 0.01, 0.05 and 0.1 μmol/L）. Real- time PCR was used to detect the expression of P-gp mRNA. Western blot assay was used to detect the expression level of P- gp. Rhodamine 123 was used to detect the efflux function level of P-gp. Cell viability and chemotherapeutic sensitivity were detected by MTT assay. Results High concentration of insulin （0.1 μmol/L） promoted the proliferation of MCF-7/ADR cells. The concentration of insulin （0.05 and 0.1 μmol/L） accelerated P-gp mRNA and protein expression, which also augmented the efflux function of P-glycoprotein and reduced the chemotherapeutic sensitivity to epirubicin. Conclusion High concentration of insulin may influence the drug resistance of breast cancer cells by promoting the expression and function of P-glycoprotein of MCF-7/ADR cells.
出处
《天津医药》
CAS
2016年第5期560-563,共4页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(81472472)
